Spheroid-based engineering of a human vasculature in mice
Spheroid-based engineering of a human vasculature in mice
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DOI：
10.1038/nmeth.1198
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发表时间：
2008-05-01
期刊：
影响因子：
48
通讯作者：
Augustin, Hellmut G.
中科院分区：
文献类型：
--
作者：
Alajati, Abdullah;Laib, Anna M.;Augustin, Hellmut G.
血管生成级联的复杂性限制了研究血管生成内皮细胞（EC）的细胞方法。反过来，体内测定不允许分析血管生成过程中 EC 的独特细胞行为。在这里，我们证明 ECs 可以作为球体移植到基质中，从而在小鼠体内产生复杂的三维人类新生血管网络。移植的脉管系统成熟并与小鼠循环相连。该测定方法用途广泛，有利于多种应用，包括研究不同细胞因子对血管生成的影响。修饰使得研究人体内淋巴管生成过程成为可能。 EC 球体还可以与其他细胞类型共同植入以用于组织工程目的。
The complexity of the angiogenic cascade limits cellular approaches to studying angiogenic endothelial cells (ECs). In turn, in vivo assays do not allow the analysis of the distinct cellular behavior of ECs during angiogenesis. Here we show that ECs can be grafted as spheroids into a matrix to give rise to a complex three-dimensional network of human neovessels in mice. The grafted vasculature matures and is connected to the mouse circulation. The assay is highly versatile and facilitates numerous applications including studies of the effects of different cytokines on angiogenesis. Modifications make it possible to study human lymphangiogenic processes in vivo. EC spheroids can also be coimplanted with other cell types for tissue engineering purposes.