Modular tissue engineering: fabrication of a gelatin-based construct
Modular tissue engineering: fabrication of a gelatin-based construct
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DOI：
10.1002/term.14
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发表时间：
2007-03-01
影响因子：
3.3
通讯作者：
Sefton, Michael V.
中科院分区：
文献类型：
--
作者：
McGuigan, Alison P.;Sefton, Michael V.
模块化组织工程构建体由亚毫米大小的圆柱形模块组装而成，这些模块接种有内皮细胞的表面层。所得到的结构被互连的内皮细胞内衬通道的网络渗透，以促进血液灌注和营养输送。为了提供足够的刚度，还包括适合内皮细胞的基质，模块化结构由明胶（胶原的变性形式）制成。使用筛分过程制造含有HepG 2细胞或球状体的明胶模块。在接种后1周内产生完全覆盖模块的牛主动脉内皮细胞表面层。将模块组装到流动回路内的结构中，并测量流速-压差曲线。即使在高流速下，液体灌注也导致可忽略的结构压实。然而，有必要用戊二醛交联明胶模块，以防止在37 ℃下溶解。这导致模块内的细胞活力显着丧失。需要能够使明胶模块无毒交联的策略来制造含有活细胞的构建体。版权所有（C）2007约翰威利父子有限公司
Modular tissue-engineered constructs are assembled from sub-millimetre-sized cylindrical modules which are seeded with a surface layer of endothelial cells. The resulting construct is permeated by a network of interconnected, endothelial cell-lined channels to facilitate blood perfusion and nutrient delivery. To provide adequate stiffness, yet consist of a substrate suitable for endothelial cells, modular constructs were fabricated from gelatin, the denatured form of collagen. Gelatin modules containing HepG2 cells or spheroids were fabricated using a sieving process. A surface layer of bovine aortic endothelial cells completely covering the modules was generated within 1 week of seeding. Modules were assembled into constructs within a flow circuit and flow rate-pressure difference profiles measured. Fluid perfusion resulted in negligible construct compaction, even at high flow rates. It was necessary, however, to crosslink the gelatin modules with glutaraldehyde to prevent dissolution at 37 degrees C. This resulted in a significant loss of cell viability within the modules. A strategy to enable non-toxic crosslinking of the gelatin modules is required to fabricate constructs containing viable cells. Copyright (C) 2007 John Wiley & Sons, Ltd.