Validation of an adapted procedure to collect hair for cortisol determination in adolescents
Validation of an adapted procedure to collect hair for cortisol determination in adolescents
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DOI：
10.1016/j.psyneuen.2016.05.002
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发表时间：
2016-08-01
影响因子：
3.7
通讯作者：
Vitaro, Frank
中科院分区：
文献类型：
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作者：
Ouellet-Morin, Isabelle;Laurin, Melissa;Vitaro, Frank
导言：在过去的几十年里，皮质醇被广泛研究与早期暴露在逆境中以及一些身体和精神问题的病因学有关。唾液和血液样本可以测量皮质醇分泌的急性变化，而头发样本被认为是长期慢性皮质醇分泌的有效回溯性测量。然而，在大型流行病学研究中，现有的头发采集方案涉及到相当大的财务和后勤挑战。目的：本研究旨在验证一种修改后的采集方案，该方案要求参与者在家中采集他们的头发，并通过普通邮件将其送回我们的实验室。他们参与了两次头发采集：(A)在他们认识的人的帮助下，在家里；(B)在我们的实验室，在一名训练有素的研究助理的帮助下。结果：我们注意到从家里收集的头发中确定的皮质醇与在实验室收集的皮质醇之间存在很强的相关性。两种方案之间的皮质醇水平没有检测到平均差异。此外，我们发现，可能与头发皮质醇水平相关的广泛的头发相关、社会人口学、生活方式因素并不影响从每个方案得出的皮质醇测量之间的关联。结论：我们的研究提供了初步支持，通过要求青少年在家中采集他们的头发样本并通过普通邮件将其发送到实验室，可以获得可靠的慢性皮质醇分泌测量。这一修改后的方案在大规模流行病学研究中具有相当大的财务和后勤优势。(C)2016爱思唯尔有限公司。保留所有权利。
Introduction: In the last decades, cortisol has been extensively studied in association to early exposure to adversity as well as in the etiology of a number of physical and mental problems. While saliva and blood samples allow the measurement of acute changes in cortisol secretion, hair samples are thought to provide a valid retrospective measure of chronic cortisol secretion over an extended period of time. Nevertheless, the existing protocol for hair collection involves considerable financial and logistical challenges when performed in large epidemiological studies.Objective: This study aimed to validate an adapted collection protocol asking participants to sample their hair at home and to send it back to our laboratory by regular mail.Methods: Participants were 34 teenagers between 17 and 18 years of age. They participated in two hair collections: (a) at home, with the help of someone they know, and (b) in our laboratory, with a trained research assistant.Results: We noted a strong correlation between cortisol ascertained from hair collected at home and at the laboratory. No mean difference in cortisol levels could be detected between the two protocols. Moreover, we showed that a wide range of hair-related, sociodemographic, lifestyle factors that may be associated with hair cortisol levels did not affect the association between cortisol measures derived from each protocol.Conclusion: Our study provides initial support that reliable measures of chronic cortisol secretion could be obtained by asking adolescents to collect a sample of their hair at home and send them to the laboratory by regular mail. This adapted protocol has considerable financial and logistical advantages in large epidemiological studies. (C) 2016 Elsevier Ltd. All rights reserved.