Culturable psychrotolerant methanotrophic bacteria in landfill cover soil
Culturable psychrotolerant methanotrophic bacteria in landfill cover soil
复制标题
垃圾填埋场覆盖土中可培养的耐冷甲烷氧化菌
作者：
A. Kallistova;L. Montonen;G. Jurgens;Uwe Münster;M. Kevbrina;A. Nozhevnikova
从4月至11月期间收集的垃圾填埋场覆盖土壤样品中，在10°C下富集的培养物中鉴定出与甲基杆菌属和甲基菌属的耐冷成员密切相关的甲烷氧化菌。在20°C下从相同的覆盖土壤样品富集的培养物中发现了甲基杆菌属和甲基弯菌属的嗜甲烷菌。从5月采集的样品（覆盖土壤温度为11.5-12.5°C）中在40°C下富集的培养物中鉴定出与Methylocaldum gracile相关的耐热甲烷氧化菌。除甲烷氧化菌外，还在富集培养物中鉴定了Methylotenera和Methylovorus属的甲基杆菌以及Verrucomicrobium、假单胞菌属、假黄单胞菌属、Dokdonella、嗜甲烷菌属、原衣原体属和硫红螺旋菌属的成员。在纯培养物中分离到一株与耐冷种地中海甲基杆菌（Methylobacter tundripaludum）（与模式菌株SV 96 T的16 S rRNA基因序列一致性为98%）密切相关的甲烷氧化菌。在填埋场覆盖土壤中引入在15°C下生长的甲烷营养富集物的混合物导致秋季（10月、11月）填埋场表面的甲烷排放量减少。所用的接种物被证明含有甲烷氧化菌密切相关的甲基杆菌tundripaludum SV 96。
Methanotrophs closely related to psychrotolerant members of the genera Methylobacter and Methylocella were identified in cultures enriched at 10°C from landfill cover soil samples collected in the period from April to November. Mesophilic methanotrophs of the genera Methylobacter and Methylosinus were found in cultures enriched at 20°C from the same cover soil samples. A thermotolerant methanotroph related to Methylocaldum gracile was identified in the culture enriched at 40°C from a sample collected in May (the temperature of the cover soil was 11.5–12.5°C). In addition to methanotrophs, methylobacteria of the genera Methylotenera and Methylovorus and members of the genera Verrucomicrobium, Pseudomonas, Pseudoxanthomonas, Dokdonella, Candidatus Protochlamydia, and Thiorhodospira were also identified in the enrichment cultures. A methanotroph closely related to the psychrotolerant species Methylobacter tundripaludum (98% sequence identity of 16S rRNA genes with the type strain SV96T) was isolated in pure culture. The introduction of a mixture of the methanotrophic enrichments, grown at 15°C, into the landfill cover soil resulted in a decrease in methane emission from the landfill surface in autumn (October, November). The inoculum used was demonstrated to contain methanotrophs closely related to Methylobacter tundripaludum SV96.