UP-REGULATION OF NITRIC-OXIDE SYNTHASE BY ESTRADIOL IN HUMAN AORTIC ENDOTHELIAL-CELLS
UP-REGULATION OF NITRIC-OXIDE SYNTHASE BY ESTRADIOL IN HUMAN AORTIC ENDOTHELIAL-CELLS
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DOI：
10.1016/0014-5793(95)00124-r
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发表时间：
1995-03-06
期刊：
影响因子：
3.5
通讯作者：
SARUTA, T
中科院分区：
文献类型：
--
作者：
HISHIKAWA, K;NAKAKI, T;SARUTA, T
我们研究了性激素对培养的人主动脉内皮细胞中钙依赖性NO生成和NO合成酶蛋白水平的影响，这些细胞用不同剂量的17 -雌二醇和睾酮处理8-48小时。17 -雌二醇处理增强了钙依赖性NO生成，但睾酮没有影响。使用单克隆抗人内皮NO合成酶抗体进行Western blot检测，结果表明17 -雌二醇处理能增加NO的生成，并伴有NO合成酶蛋白的升高。我们的结果提供了证据，证明人内皮NO合酶可以被雌激素调节。
We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment,vas accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.