The "FERMEX" method for metabolite-enriched fungal extracts.
The "FERMEX" method for metabolite-enriched fungal extracts.
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DOI：
10.1007/978-1-62703-122-6_5
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Goetz, Michael A
中科院分区：
文献类型：
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作者：
Bills, Gerald F;Dombrowski, Anne W;Goetz, Michael A
FERMEX（发酵提取物）计划是一个非常成功的微生物天然产物分子来源，用于药物先导发现。该计划是基于这样的观察，即真菌的固体发酵通常表现出比相同菌株在液体培养基上生长时更复杂的代谢产物谱。为了产生无干扰的发酵和提高有机产品的回收，真菌定殖均匀介质饱和蛭石，从而促进细胞和代谢分化。用甲基乙基酮从基质和培养基中提取真菌细胞中的次级代谢产物，以产生富含代谢物的筛选样品。必要的设备，协议，和媒体配方描述沿着与生物活性的真菌代谢产物在这个系统中产生的例子。
The FERMEX (Fermentation Extract) program was a highly successful source of microbial natural product molecules for pharmaceutical lead discovery. The program was based on the observation that solid fermentations of fungi generally exhibited more complex metabolite profiles than when the same strains were grown on liquid medium. To produce interference-free fermentations and improve organic product recovery, fungi colonized homogeneous media-saturated vermiculite thus promoting cellular and metabolic differentiation. Secondary metabolites in fungal cells were extracted from the substratum and medium with methyethylketone to generate metabolite-enriched screening samples. The necessary equipment, protocol, and media recipes are described along with examples of bioactive fungal metabolites produced in this system.