The separation and characterization of the methylumbelliferyl beta-galactosidases of human liver.
The separation and characterization of the methylumbelliferyl beta-galactosidases of human liver.
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人肝脏甲基伞形基β-半乳糖苷酶的分离和表征。
DOI：
10.1042/bj1570189
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
N. Dance
中科院分区：
文献类型：
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作者：
P. Cheetham;N. Dance
1.通过凝胶过滤分离了一种以前未表征的人肝酸性β-半乳糖苷酶（EC 3.2.1.23），可能是分子量为160 000的二聚体。它具有与其他两种酸性β-半乳糖苷酶形式相同的水解GM 1神经节苷脂的能力。2.酸性β-半乳糖苷酶的低分子量形式经历盐依赖性聚集。3.高分子量组分可以由结合到膜碎片的低分子量形式组成。它可以完全转化为这些形式的混合物。4.中性β-半乳糖苷酶活性可通过DEAE-纤维素层析分离为两种形式。它们对Cl-离子的反应不同。5.本文提出了一种新的命名法，用于迄今为止在人类肝脏中发现的六种β-半乳糖苷酶。6.对电泳分离的β-半乳糖苷酶带的酶组分进行了重新检查。A带包含三个分量。一个二维电泳程序解决A带的描述。7.研究了神经氨酸酶处理对β-半乳糖苷酶在各种分离系统中的行为的影响。
1. A previously uncharacterized form of human liver acid beta-galactosidase (EC 3.2.1.23), possibly a dimer of molecular weight 160 000, was resolved by gel filtration. It has the same ability to hydrolyse GM1 ganglioside as the two other acid beta-galactosidase forms. 2. The low-molecular-weight forms of acid beta-galactosidase undergo salt-dependent aggregation. 3. The high-molecular-weight component may consist of the low-molecular-weight forms bound to membrane fragments. It can be converted completely into a mixture of these forms. 4. The neutral beta-galactosidase activity can be resolved into two forms by DEAE-cellulose chromatography. They differ in their response to Cl-ions. 5. A new nomenclature is suggested for the six beta-galactosidases so far found in human liver. 6. The enzymic constituents of the beta-galactosidase bands resolved by electrophoresis were re-examined. The A band contains three components. A two-dimensional electrophoretic procedure for resolving the A band is described. 7. The effect of neuraminidase treatment on the behaviour of beta-galactosidases in various separation systems is examined.