Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma
Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma
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DOI：
10.1016/j.bmcl.2013.01.040
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发表时间：
2013-03-15
影响因子：
2.7
通讯作者：
Shimizu, Nobuaki
中科院分区：
文献类型：
--
作者：
Ninomiya, Kazuaki;Kaneda, Kazuhiko;Shimizu, Nobuaki
识别人肝癌的单链 DNA 适体是通过使用全细胞作为靶标（cell-SELEX）进行指数富集的配体系统进化来分离的。以人肝癌HepG2细胞为靶标，人正常肝细胞为对照，经过11轮cell-SELEX程序，获得12个独立的DNA适配体候选序列。确认了选定的DNA适体和靶细胞之间的特异性相互作用。所获得的 12 个序列的解离常数也估计在 19-450 nM 范围内。此外，在分离的适体中发现了共有的二级结构，该结构负责HepG2细胞的识别。 (C) 2013 Elsevier Ltd. 保留所有权利。
Single-stranded DNA aptamers recognizing human hepatocarcinoma were isolated by means of a systematic evolution of ligands by exponential enrichment using whole cells as targets (cell-SELEX). After 11 rounds of cell-SELEX procedure using human hepatoma HepG2 cells as targets and human normal hepatocyte cells as counterparts, 12 independent DNA aptamer candidate sequences were obtained. The specific interaction between selected DNA aptamers and targeted cell was confirmed. Dissociation constants of the 12 sequences obtained were also estimated in the range of 19-450 nM. Moreover, the consensus secondary structure was found in the isolated aptamers, which was responsible to the recognition of HepG2 cells. (C) 2013 Elsevier Ltd. All rights reserved.