Capillary electrophoresis - Mass spectrometry metabolomics analysis revealed enrichment of hypotaurine in rat glioma tissues
Capillary electrophoresis - Mass spectrometry metabolomics analysis revealed enrichment of hypotaurine in rat glioma tissues
复制标题
毛细管电泳-质谱代谢组学分析揭示大鼠神经胶质瘤组织中亚牛磺酸的富集
DOI：
10.1016/j.ab.2017.08.012
复制
发表时间：
2017-11-15
影响因子：
2.9
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Gao, Peng;Ji, Min;Zhang, Yong
脑胶质瘤是一种致命性最强的脑部恶性肿瘤，其病因不明。针对不同种类的样品进行了多种代谢组学分析。由于采用了不同的分析平台，所报道的与疾病相关的代谢物在不同研究中并不一致。具有可比性的代谢组学结果更有可能通过使用相同的分析平台分析相同的样本类型而获得。对于肿瘤研究来说，组织样本代谢组学分析具有独特的优势，它可以更直接地了解疾病特异性的病理分子。鉴于此，我们采用了一种先前报道的毛细管电泳质谱人组织代谢组学分析方法，对大鼠C6细胞移植瘤及相应的癌前组织的代谢组进行了分析。结果发现，脑胶质瘤组织中有9种代谢物增加。其中，亚牛磺酸是唯一一种在人体相关组织代谢组学分析中报道的富含在肿瘤组织中的代谢物。此外，通过免疫细胞化学染色和C6细胞培养的体外酶活性测定，证实了亚牛磺酸对α-酮戊二酸依赖的双加氧酶(2-KDD5)的抑制作用，证实了亚牛磺酸是2-KDDs的竞争性抑制剂的结论，证明了代谢组学在肿瘤学研究中的价值。(C)爱思唯尔公司出版的2017年。
Glioma is one of the most lethal brain malignancies with unknown etiologies. Many metabolomics analysis aiming at diverse kinds of samples had been performed. Due to the varied adopted analytical platforms, the reported disease-related metabolites were not consistent across different studies. Comparable metabolomics results are more likely to be acquired by analyzing the same sample types with identical analytical platform. For tumor researches, tissue samples metabolomics analysis own the unique advantage that it can gain more direct insight into disease-specific pathological molecules. In this light, a previous reported capillary electrophoresis mass spectrometry human tissues metabolomics analysis method was employed to profile the metabolome of rat C6 cell implantation gliomas and the corresponding precancerous tissues. It was found that 9 metabolites Increased in the glioma tissues. Of them, hypotaurine was the only metabolite that enriched in the malignant tissues as what had been reported in the relevant human tissues metabolomics analysis. Furthermore, hypotaurine was also proved to inhibit alpha-ketoglutarate-dependent dioxygenases (2-KDD5) through immunocytochemistry staining and in vitro enzymatic activity assays by using C6 cell cultures, This study reinforced the previous conclusion that hypotaurine acted as a competitive inhibitor of 2-KDDs and proved the value of metabolomics in oncology studies. (C) 2017 Published by Elsevier Inc.