Investigation of the 1994-5 Ukrainian Vibrio cholerae epidemic using molecular methods
Investigation of the 1994-5 Ukrainian Vibrio cholerae epidemic using molecular methods
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DOI：
10.1017/s0950268898008814
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发表时间：
1998-08-01
影响因子：
4.2
通讯作者：
Johnson, WM
中科院分区：
文献类型：
--
作者：
Clark, CG;Kravetz, AN;Johnson, WM
采用分子方法分析了乌克兰分离的37株霍乱弧菌和4株非霍乱弧菌，包括1994-5年流行的菌株。PFGE和核分型结果表明，乌克兰产毒霍乱弧菌相互之间及与巴基斯坦1例患者分离的霍乱弧菌亲缘关系较近，在疫情高峰期获得的一株非产毒霍乱弧菌与这些霍乱弧菌亲缘关系较远，而副溶血性弧菌分离株和溶藻弧菌分离株与霍乱弧菌亲缘关系或彼此之间均不密切。ERIC- pcr和REP-PCR可以区分与其他方法相同的菌株。所获得的结果证实，乌克兰流行毒株与来自亚洲的第七次大流行毒株最密切相关，并支持1994-5年乌克兰流行毒株是由自1991年乌克兰流行以来或之前存活的产毒环境毒株引起的假设。
Thirty-seven Vibrio cholerae and four non-cholera Vibrio isolates from Ukraine, including strains from the epidemic of 1994-5, were analysed by molecular methods. Results from PFGE and ribotyping indicated that all Ukrainian toxigenic V. cholerae were closely related to each other and to an isolate from a patient from Pakistan, A non-toxigenic river water strain obtained during the height of the epidemic was more distantly related to these V. cholerae strains, while the Vibrio parahaemolyticus isolates and Vibrio alginolyticus isolate were not closely related to V. cholerae or each other. ERIC- and REP-PCR allowed the differentiation of strains identical by other methods. The results obtained confirm that the epidemic Ukrainian strains are most closely related to seventh pandemic strains from Asia and support a hypothesis that the Ukrainian epidemic of 1994-5 was caused by toxigenic environmental strains surviving since the time of the 1991 Ukrainian epidemic or before.