Development of an automated sampling-analysis system for simultaneous measurement of reactive oxygen species (ROS) in gas and particle phases: GAC-ROS
Development of an automated sampling-analysis system for simultaneous measurement of reactive oxygen species (ROS) in gas and particle phases: GAC-ROS
复制标题
开发自动采样分析系统，用于同时测量气相和颗粒相中的活性氧 (ROS)：GAC-ROS
DOI：
10.1016/j.atmosenv.2016.03.038
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发表时间：
2016-06-01
影响因子：
5
通讯作者：
Schauer, James J.
中科院分区：
文献类型：
--
作者：
Huang, Wei;Zhang, Yuanxun;Schauer, James J.
基于2‘，7’-二氯荧光素(DCFH)分析方法，建立了一种可同时在线测定气相和颗粒相中ROS的新型在线系统GAC-ROS，用于快速采样和分析大气中的ROS。GAC-ROS由气体和气溶胶收集器(GAC)、一系列反应和输送系统以及荧光检测器组成，在实验室进行了仪器性能测试。结果表明，该方法具有良好的校正曲线R-2值(0.998以上)、较高的ROS穿透效率(99.5%以上)和较低的检测下限(气相ROS：0.16nmolH_2O_2 m(-3)；颗粒相ROS：0.12nmol H_2O_2 m(-3))。通过对在线和离线两种ROS检测方法的实验室比较发现，由于离线处理时间较长，ROS的损失较大。北京的野外观测发现，冬季的ROS浓度明显高于春季的观测结果。ROS与部分大气污染物之间只有少量的弱正相关，反映了大气中ROS产生和转化的复杂性。这项研究首次使用在线方法同时获得了气相和颗粒相的ROS浓度。因此，它为表征大气的氧化能力和氧化能力的来源提供了一个强有力的工具。(C)爱思唯尔有限公司出版的2016年。
A novel online system, GAC-ROS, for simultaneous measurement of reactive oxygen species (ROS) in both gas and particle phases was developed based on 2',7'-dichlorofluorescin (DCFH) assay to provide fast sampling and analysis of atmospheric ROS. The GAC-ROS, composed of a Gas and Aerosol Collector (GAC), a series of reaction and transportation systems, and a fluorescence detector, was tested for instrumental performance in laboratory. Results showed good performance with a favorable R-2 value for the calibration curve (above 0.998), high penetration efficiencies of ROS (above 99.5%), and low detection limits (gas-phase ROS: 0.16 nmol H2O2 m(-3); particle-phase ROS: 0.12 nmol H2O2 m(-3)). Laboratorial comparison between online and offline methods for particle-bound ROS showed significant loss of ROS due to the relatively long time off-line treatment. Field observations in Beijing found that concentrations of ROS in winter time were significantly higher than those observed in spring. Only a few weak positive correlations were found between ROS and some air pollutants, which reflects the complexities of ROS generation and transformation in atmosphere. This study was the first to simultaneously obtain concentrations of gas and particle-phase ROS using an online method. Consequently, it provides a powerful tool to characterize the oxidizing capacity of the atmosphere and the sources of the oxidizing capacity. (C) 2016 Published by Elsevier Ltd.