Hyperbaric oxygenation enhances transplanted cell graft and functional recovery in the infarct heart.
Hyperbaric oxygenation enhances transplanted cell graft and functional recovery in the infarct heart.
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DOI：
10.1016/j.yjmcc.2009.04.005
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发表时间：
2009-08
影响因子：
5
通讯作者：
Kuppusamy, Periannan
中科院分区：
文献类型：
--
作者：
Khan, Mahmood;Meduru, Sarath;Mohan, Iyyapu K.;Kuppusamy, M. Lakshmi;Wisel, Sheik;Kulkarni, Aditi;Rivera, Brian K.;Hamlin, Robert L.;Kuppusamy, Periannan
关键词：
A major limitation to the application of stem-cell therapy to repair ischemic heart damage is the low survival of transplanted cells in the heart, possibly due to poor oxygenation. We hypothesized that hyperbaric oxygenation (HBO) can be used as an adjuvant treatment to augment stem-cell therapy. Therefore, the goal of this study was to evaluate the effect of HBO on the engraftment of rat bone-marrow-derived mesenchymal stem cells (MSCs) transplanted in infarct rat hearts. Myocardial infarction (MI) was induced in Fisher-344 rats by permanently ligating the left-anterior-descending coronary artery. MSCs, labeled with fluorescent superparamagnetic iron oxide (SPIO) particles, were transplanted in the infarct and peri-infarct regions of the MI hearts. HBO (100% oxygen at 2 ATA for 90 min) was administered daily for 2 weeks. Four MI groups were used: untreated (MI); HBO; MSC; MSC+HBO. Echocardiography, electro-vectorcardiography, and magnetic resonance imaging were used for functional evaluations. The engraftment of transplanted MSCs in the heart was confirmed by SPIO fluorescence and Prussian-blue staining. Immunohistochemical staining was used to identify key cellular and molecular markers including CD29, troponin-T, connexin-43, VEGF, α-smooth-muscle actin, and von-Willebrand factor in the tissue. Compared to MI and MSC groups, the MSC+HBO group showed a significantly increased recovery of cardiac function including left-ventricular (LV) ejection fraction, fraction-shortening, LV wall-thickness, and QRS vector. Further, HBO treatment significantly increased the engraftment of CD29-positive cells, expression of connexin-43, troponin-T and VEGF, and angiogenesis in the infarct tissue. Thus, HBO appears to be a potential and clinically-viable adjuvant treatment for myocardial stem-cell therapy.
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影响因子：
1.9
作者：
Stavitsky, Y;Shandling, AH;Clarke, D
通讯作者：
Clarke, D
影响因子：
4.6
作者：
Al-Khaldi, A;Al-Sabti, H;Lachapelle, K
通讯作者：
Lachapelle, K
影响因子：
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Ashraf, Muhammad
影响因子：
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作者：
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通讯作者：
Sun, Xuejun
DOI：
10.1152/ajpheart.00677.2007
发表时间：
2007-10-01
影响因子：
4.8
作者：
Khan, Mahmood;Kutala, Vijay Kumar;Kuppusamy, Periannan
通讯作者：
Kuppusamy, Periannan