Characterization of the recognition of blood group B trisaccharide derivatives by the lectin from Marasmius oreades using frontal affinity chromatography-mass spectrometry
Characterization of the recognition of blood group B trisaccharide derivatives by the lectin from Marasmius oreades using frontal affinity chromatography-mass spectrometry
复制标题
DOI：
10.1023/a:1024297623445
复制
发表时间：
2002-03-01
影响因子：
3
通讯作者：
Hindsgaul, O
中科院分区：
文献类型：
--
作者：
Rempel, BP;Winter, HC;Hindsgaul, O
来自蘑菇Marasmius oreades（MOA）的一种新型凝集素已被证明与人血型B寡糖结合[1]。在目前的工作中，我们研究了血型B-三糖的一系列类似物alphaGal（1-3）[alphaFuc（1-2）] betaGal-OR（1，R =（CH 2）（8）COOMe）的结合。将MOA生物素化并固定在微柱（9.8穆尔）上，以通过正面亲和色谱-质谱法（FAC-MS）进行评价[2]。发现三糖1是最大识别所需的表位（Kd = 3.6 μ M）。一系列的合成脱氧和O-甲基化的类似物的B-三糖（R = OMe），然后筛选对凝集素，并确定结合的关键结构元素。发现β-Gal残基的OH-4和α-Gal残基的OH-2对于识别至关重要。FAC-MS技术在评价化合物的混合物方面也被证明是强大的。由于B-三糖的溶液NMR结构和晶体结构是已知的[3]，我们提出了被凝集素识别的三糖的比表面。2003年出版。
A novel lectin from the mushroom Marasmius oreades (MOA) has been shown to bind to human blood group B oligosaccharides [1]. In the present work we examine the binding of a series of analogues of the blood group B-trisaccharide, alphaGal(1-3)[alphaFuc(1-2)]betaGal-OR (1, R = (CH2)(8)COOMe). MOA was biotinylated and immobilized on a micro column (9.8 muL) for evaluation by Frontal Affinity Chromatography-Mass Spectrometry (FAC-MS) [2]. The trisaccharide 1 was found to be the epitope needed for maximum recognition (K-d = 3.6 muM). A series of synthetic deoxygenated and O-methylated analogues of the B-trisaccharide (R = OMe) were then screened against the lectin, and the key structural elements for binding were determined. OH-4 of the beta-Gal residue and OH-2 of the a-Gal residue were found to be critical for recognition. The FAC-MS technique also proved powerful in evaluating mixtures of compounds. Since the solution NMR structure and crystal structure of the B-trisaccharide are known [3], we propose the specific surface of the trisaccharide that is recognized by the lectin. Published in 2003.