The induction and possible subsequent effect of human antibodies against porcine enamel matrix derivative.
The induction and possible subsequent effect of human antibodies against porcine enamel matrix derivative.
复制标题
人类抗体针对猪釉质基质衍生物的诱导和可能的后续作用。
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
W. Tsai
中科院分区：
文献类型：
--
作者：
K. Yuan;Chia;W. Tsai
背景
猪釉原蛋白是牙釉质基质衍生物 (EMD) 的主要成分，其氨基酸序列与其人类对应物的氨基酸序列大约有 91% 相同。猪 EMD (pEMD) 是否可以在第一次手术后引发中和抗体，从而降低二次手术的临床效果，目前尚未确定。
方法
在手术前后收集接受或不进行 pEMD 的牙周手术患者的血清。将 pEMD 产物进行电泳并转移用于使用来自患者的纯化抗体作为一抗进行蛋白质印迹。为了阐明患者体内的抗体是否可以抑制转化生长因子-β1 (TGF-β1) 的产生，我们将不同量的 pEMD 患者（手术前与手术后）的纯化抗体添加到牙周膜成纤维细胞的细胞培养物中。然后收集条件培养基用于TGF-β1的免疫测定。
结果
蛋白质印迹结果表明，手术后 10 天就产生了针对 pEMD 的人类抗体。使用质谱分析，蛋白质印迹上的非特异性条带似乎是猪免疫球蛋白 G (IgG)。免疫测定结果显示，接受 pEMD 治疗的患者的抗体不会阻碍后续 TGF-β1 的产生。
结论
pEMD 产品被猪 IgG 污染。该产品的应用可以在人体中诱导针对猪牙釉蛋白不同异构体的抗体。然而，增加的抗体并不会阻碍 TGF-β1 的产生，TGF-β1 是 pEMD 对牙周膜成纤维细胞的体外功能之一。
BACKGROUND
The amino acid sequence of porcine amelogenin, the major component of enamel matrix derivative (EMD), is approximately 91% identical to that of its human counterpart. Whether porcine EMD (pEMD) can elicit neutralizing antibodies after the first surgery, thereby reducing the clinical effect of secondary surgery, has not been established.
METHODS
The sera of patients receiving periodontal surgery with or without pEMD were collected before and after surgery. The pEMD product was subjected to electrophoresis and transferred for a Western blot using the purified antibodies from patients as the primary antibodies. To clarify whether the antibodies in patients could inhibit the production of transforming growth factor-beta1 (TGF-beta1), we added different amounts of purified antibodies from pEMD patients (before versus after surgery) into the cell cultures of periodontal ligament fibroblasts. The conditioned media were then collected for an immunoassay of TGF-beta1.
RESULTS
The result of the Western blot demonstrated that human antibodies against pEMD were elicited 10 days after surgery. Using mass spectrometry, a non-specific band on the Western blot appeared to be porcine immunoglobulin G (IgG). The results of the immunoassay showed the antibodies from pEMD-treated patients did not hinder the subsequent production of TGF-beta1.
CONCLUSIONS
The pEMD product was contaminated with porcine IgG. The application of the product could induce antibodies against different isoforms of porcine amelogenin in humans. However, the increased antibodies did not hinder the production of TGF-beta1, one of the established in vitro functions of pEMD on periodontal ligament fibroblasts.
食物过敏儿童口服免疫球蛋白制剂的过敏性。
影响因子：
8
作者：
Bernhisel-Broadbent,J;Yolken,RH;Sampson,HA
通讯作者：
Sampson,HA
牛 IgG 可以在模拟巴氏灭菌的条件下聚集并与一些人 Fc γ 受体结合。
影响因子：
3.6
作者：
KulczyckiJr,A
通讯作者：
KulczyckiJr,A