Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse
Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse
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DOI：
10.1007/s00429-016-1303-0
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发表时间：
2017-05-01
影响因子：
3.1
通讯作者：
Itoi, Keiichi
中科院分区：
文献类型：
--
作者：
Kono, Junko;Konno, Kohtarou;Itoi, Keiichi
我们研究了促肾上腺皮质激素释放因子（CRF）神经元的形态学特征，在一个小鼠系中，修饰的黄色荧光蛋白（金星）下的CRF启动子表达。我们先前产生了CRF-金星敲入小鼠，其中金星通过同源重组插入CRF基因位点。在本研究中，由pgk-1启动子驱动的新霉素磷酸转移酶基因（Neo）从CRF-Venus小鼠基因组中缺失，并产生CRF-Venusa匕首Neo小鼠。当与CRF-Venus小鼠相比时，Venus表达在CRF-Venusa匕首Neo小鼠中显著得多。此外，大多数表达Venus的神经元共表达CRF mRNA。表达静脉的神经元构成了下丘脑室旁核（PVH）中投射到正中隆起的神经内分泌神经元的离散群体。在神经内分泌PVH以外的脑区也发现了静脉表达神经元，包括嗅球、梨状皮质（Pir）、延伸杏仁核、海马、新皮质、巴林顿核、中脑/脑桥背侧被盖、导水管周围灰质和下橄榄核（IO）。即使在CRF免疫反应阳性的胞体难以识别的区域，也可以清楚地观察到共表达CRF mRNA的表达Venus的胞体。我们证明，CRF神经元含有谷氨酸的PIR和IO，而它们含有γ-氨基丁酸的新皮层，床核的终纹，海马和杏仁核。CRF神经元的人口被证明是胆碱能在中脑被盖。CRF-Venusa dagger Neo小鼠可用于研究小鼠脑中CRF神经元的结构和功能特性。
We examined the morphological features of corticotropin-releasing factor (CRF) neurons in a mouse line in which modified yellow fluorescent protein (Venus) was expressed under the CRF promoter. We previously generated the CRF-Venus knock-in mouse, in which Venus is inserted into the CRF gene locus by homologous recombination. In the present study, the neomycin phosphotransferase gene (Neo), driven by the pgk-1 promoter, was deleted from the CRF-Venus mouse genome, and a CRF-Venusa dagger Neo mouse was generated. Venus expression is much more prominent in the CRF-Venusa dagger Neo mouse when compared to the CRF-Venus mouse. In addition, most Venus-expressing neurons co-express CRF mRNA. Venus-expressing neurons constitute a discrete population of neuroendocrine neurons in the paraventricular nucleus of the hypothalamus (PVH) that project to the median eminence. Venus-expressing neurons were also found in brain regions outside the neuroendocrine PVH, including the olfactory bulb, the piriform cortex (Pir), the extended amygdala, the hippocampus, the neocortices, Barrington's nucleus, the midbrain/pontine dorsal tegmentum, the periaqueductal gray, and the inferior olivary nucleus (IO). Venus-expressing perikarya co-expressing CRF mRNA could be observed clearly even in regions where CRF-immunoreactive perikarya could hardly be identified. We demonstrated that the CRF neurons contain glutamate in the Pir and IO, while they contain gamma-aminobutyric acid in the neocortex, the bed nucleus of the stria terminalis, the hippocampus, and the amygdala. A population of CRF neurons was demonstrated to be cholinergic in the midbrain tegmentum. The CRF-Venusa dagger Neo mouse may be useful for studying the structural and functional properties of CRF neurons in the mouse brain.