[Methodological errors in amino acid assay in biological fluids].
[Methodological errors in amino acid assay in biological fluids].
复制标题
[生物体液中氨基酸测定的方法学错误]。
DOI：
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发表时间：
1991
影响因子：
0.5
通讯作者：
P. Kamoun
中科院分区：
文献类型：
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作者：
P. Parvy;J. Bardet;D. Rabier;P. Kamoun
在生物体液中游离氨基酸的测定中观察到了许多方法上的错误。其中一些与用于血液采样的试管或抗凝剂中存在或形成氨基酸或吲哚三酮阳性化合物有关。溶血或凝血改变了血浆中某些氨基酸的浓度。在毛细血管血中观察到的一个特殊误差与皮肤区域存在的氨基酸有关。用于分析的尿液或缓冲液的细菌污染也会影响氨基酸分析的结果。
Numerous methodological errors have been observed in the measurement of free-amino acids in the biological fluids. Some of them are related to the presence or the formation of amino acids or ninhydrin-positive compounds in tubes or anticoagulants used for the blood sampling. Hemolysis or blood clotting modify the concentration of some amino acids in plasma. A special error observed with the capillary blood is related to the amino acids present on area of the skin. Bacterial contamination of urines or buffers used for the analysis can also modify the results of the amino acids assays.