On the Promising Role of Enzyme Activity Assay in Interpreting Comparative Proteomic Data in Plants
On the Promising Role of Enzyme Activity Assay in Interpreting Comparative Proteomic Data in Plants
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酶活性测定在解释植物比较蛋白质组数据中的前景广阔
DOI：
10.1002/pmic.201800234
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发表时间：
2018
期刊：
影响因子：
3.4
通讯作者：
Wei Wang
中科院分区：
文献类型：
--
作者：
Liangjie Niu;Xiangru Xu;Hui Liu;Xiaolin Wu;Wei Wang
比较蛋白质组学被广泛用于检测蛋白质的变化，特别是差异丰度蛋白（DAP），这些蛋白质参与植物对发育、疾病或环境的反应。一旦确定了DAP，就必须验证其丰度的任何变化及其在所研究的生物过程中的作用。除了通过定量RT-PCR、免疫印迹和多反应监测分析进行确认外，还提出酶活性测定（EAA）可以补充标准蛋白质组学结果，特别是在阐明蛋白质（酶）功能和酶相关生化或代谢途径的机制方面。这里讨论的酶是比较植物蛋白质组学中鉴定的DAP。尽管蛋白质组中的酶数量很少，但它们通常构成比较研究中鉴定的DAP的相当大的比例。目前，只有少数研究进行了EAA，以补充蛋白质组学数据的解释，特别是基于活性的蛋白质分析。这一观点旨在引起蛋白质组学研究人员对EAA在植物蛋白质组学中的潜在作用的关注，并强调在比较植物蛋白质组学中需要高通量的酶活性测定。
Comparative proteomics is widely used to detect protein changes, especially differential abundance proteins (DAPs) that are involved in plant responses to development, disease, or environment. Once DAPs are identified, it is essential to validate any change in their abundance, and their role in the biological process under study. In addition to common confirmation by quantitative RT‐PCR, immunoblot, and multiple reaction monitoring analysis, it has been proposed that enzyme activity assay (EAA) can be complementary to the standard proteomics results, especially regarding the elucidation of protein (enzyme) function and the mechanism of enzyme‐associated biochemical or metabolic pathways. The enzymes discussed here are the DAPs identified in comparative plant proteomics. Despite the small number of enzymes in a proteome, they often make up a substantial proportion of the DAPs identified in comparative studies. Currently, only a few studies have performed EAA to complement the interpretation of proteomic data, especially activity‐based protein profiling. This viewpoint aims to arouse the attention of proteomic researchers on the promising role of EAA in plant proteomics and highlights the need for high‐throughput assays of enzyme activities in comparative plant proteomics.