Cellular Endocytosis and Trafficking of Cholera Toxin B-Modified Mesoporous Silica Nanoparticles.
Cellular Endocytosis and Trafficking of Cholera Toxin B-Modified Mesoporous Silica Nanoparticles.
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DOI：
10.1039/c5tb02079d
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发表时间：
2016-02-21
期刊：
影响因子：
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通讯作者：
Vivero-Escoto JL
中科院分区：
文献类型：
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作者：
Walker WA;Tarannum M;Vivero-Escoto JL
在这项研究中，介孔二氧化硅纳米颗粒 (MSN) 通过霍乱毒素 B 亚基 (CTxB) 蛋白进行功能化，以影响其细胞内运输途径。合成了CTxB-MSN载体，并对其化学和结构性质进行了表征。内吞途径抑制测定表明，人宫颈癌 (HeLa) 细胞对 CTxB-MSN 的摄取部分受到 Chlatrin 和小窝介导的内吞作用机制的促进。激光扫描共焦显微镜 (LSCM) 实验表明，CTxB-MSN 被细胞摄取，并部分通过跨高尔基体网络以逆行方式运输到内质网。使用碘化丙啶（一种不可渗透的细胞膜染料）评估 CTxB-MSN 的递送能力。 LSCM 图像描绘了 HeLa 细胞内质网和细胞核中碘化丙啶的释放。
In this study, mesoporous silica nanoparticles (MSNs) were functionalized with Cholera toxin subunit B (CTxB) protein to influence their intracellular trafficking pathways. The CTxB-MSN carrier was synthesized, and its chemical and structural properties were characterized. Endocytic pathway inhibition assays showed that the uptake of CTxB-MSNs in human cervical cancer (HeLa) cells was partially facilitated by both chlatrin- and caveolae-mediated endocytosis mechanisms. Laser scanning confocal microscopy (LSCM) experiments demonstrated that CTxB-MSNs were taken up by the cells and partially trafficked through the trans-Golgi network into to the endoplasmic reticulum in a retrograde fashion. The delivery abilities of CTxB-MSNs were evaluated using propidium iodide, an impermeable cell membrane dye. LSCM images depicted the release of propidium iodide in the endoplasmic reticulum and cell nucleus of HeLa cells.