A Method for Manufacturing Membranes with Ultrathin Hydrogel Selective Layers for Protein Purification: Interfacially Initiated Free Radical Polymerization (IIFRP)
A Method for Manufacturing Membranes with Ultrathin Hydrogel Selective Layers for Protein Purification: Interfacially Initiated Free Radical Polymerization (IIFRP)
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DOI：
10.1021/acs.chemmater.7b04598
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发表时间：
2018-02-27
影响因子：
8.6
通讯作者：
Asatekin, Ayse
中科院分区：
文献类型：
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作者：
Sadeghi, Ilin;Yi, Hyunmin;Asatekin, Ayse
水凝胶具有耐污染、可调的网目尺寸和可功能化等特点，是一种很有前途的膜选择层材料。这些特性对于蛋白质纯化应用尤为重要。然而，使用简单、可扩展的工艺制备薄而均匀的水凝胶膜选择性层是一项尚未解决的挑战。我们展示了一种新的方法，界面引发自由基聚合(IIFRP)，以一种简单且可重复的过程在多孔载体上制备超薄水凝胶选择性层。这种方法利用单体和光引发剂分离成两个独立的、不相容的相，在光聚合时在界面形成非常薄、均匀和无缺陷的水凝胶层。由此产生的水凝胶涂层膜具有薄至
Hydrogels are promising materials as membrane selective layers due to their fouling-resistant nature, tunable mesh size, and functionalizability. These features are especially critical for protein purification applications. However, the fabrication of thin, uniform hydrogel membrane selective layers using a simple, scalable process is an unmet challenge. We demonstrate a new method, interfacially initiated free radical polymerization (IIFRP), for fabrication of ultrathin hydrogel selective layers on porous supports in a simple and reproducible process. This method utilizes segregation of the monomer and the photoinitiator into two separate, immiscible phases to form a very thin, uniform, and defect-free hydrogel layer at the interface upon photopolymerization. The resulting hydrogel-coated membranes have selective layers as thin as