Effects of Extracellular Matrix on Mechanical Properties of Articular Cartilage.
Effects of Extracellular Matrix on Mechanical Properties of Articular Cartilage.
复制标题
细胞外基质对关节软骨机械性能的影响。
DOI：
10.1299/kikaia.69.84
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
K. Ikeuchi
中科院分区：
文献类型：
--
作者：
Y. Morita;N. Tomita;H. Aoki;S. Takeda;K. Sakaguchi;K. Ikeuchi
本研究旨在探讨细胞外基质与关节软骨力学性能的相关性。胶原酶和透明质酸酶分别用于降解胶原纤维和蛋白多糖。通过压痕试验、动态粘弹性测试和拉伸试验对酶处理前后的力学性能进行了评价。压痕试验结果表明，随着处理时间的延长，两种酶处理的最大应变均增加，而回复率（=回复应变/最大应变）均降低。透明质酸酶处理前后的拉伸强度无显著差异，而胶原酶处理后的拉伸强度降低。动态粘弹性测量结果显示，透明质酸酶处理后，tan δ的峰值和峰值频率随处理时间的推移而向高值移动。这些结果表明，压缩模量和粘弹性性能的胶原蛋白多糖网络的影响和拉伸强度主要是由胶原纤维。
The purpose of this study is to evaluate correlation between extracellular matrix and mechanical properties of articular cartilage. Collagenase and hyaluronidase were used to degrade colloagen fibril and proteoglycan respectively. Indentation test, dynamic visco-elasticity measurement and tensile test were carried out to evaluate mechanical properties before and after the enzyme treatment. Acoording to indentation test, maximum strain was increased and recovery ratio (=recovery strain/maximum strain) was decreased with treatment time for both enzyme treatments. There were no significant differences in tensile strength before and after the hyaluronidase treatment, where as the strength was decreased by the collgenase treatment. Result of dynamic visco-elasticity measurement showed that peak value and peak frequency of tan δ were shifted to a higher value with treatment time after the hyaluronidase treatment. These results suggest that compressive modulus and visco-elastic properties are much affected by collagen-proteoglycan network and tensile strength is affected mainly by collagen fibers.