The expression of collagen I and XII mRNAs in Porphyromonas gingivalis-induced periodontitis in rats:: The effect of doxycycline and chemically modified tetracycline
The expression of collagen I and XII mRNAs in Porphyromonas gingivalis-induced periodontitis in rats:: The effect of doxycycline and chemically modified tetracycline
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DOI：
10.1902/jop.1998.69.1.34
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发表时间：
1998-01-01
影响因子：
4.3
通讯作者：
Nishimura, I
中科院分区：
文献类型：
--
作者：
Karimbux, NY;Ramamurthy, NS;Nishimura, I
组织重塑是一种动态状态，其中细胞外基质的蛋白水解分解和合成之间实现平衡。 I 型胶原蛋白是牙龈结缔组织 (GCT) 和牙周膜 (PDL) 整个发育过程中的主要成分，而 XII 型胶原蛋白则存在于这些组织的成熟形式中。本研究的目的是研究牙周炎对 I 型和 XII 型胶原蛋白表达的影响，并随后研究多西环素 (DOXY) 和化学修饰的非抗菌四环素 (CMT-1) 对模型中这些分子表达的影响。成年障碍饲养的雄性斯普拉格-道利大鼠接种了从人类身上获得的牙龈卟啉单胞菌，以产生实验性牙周炎。然后将患有牙龈卟啉单胞菌诱发的牙周炎的动物分为以下几组：A组作为感染的未治疗对照（PGI组）； B组采用多西环素治疗(DOXY组); C组采用化学修饰四环素-1治疗(CMT-1组)。 D 组包含未感染的动物，作为未感染的对照（NIC 组）。通过原位杂交检测各组中 I 型和 XII 型胶原 mRNA 的表达，这些分子的共表达代表成熟且有功能的牙龈结缔组织。在NIC组中，发现与编码大鼠α2(I)或α1(XII)胶原蛋白的地高辛标记的cDNA探针杂交的细胞均匀分布在整个牙周结缔组织中。 PGI组在感染区域几乎没有表现出杂交，而DOXY和CMT-1组都在GCT和PDL的冠状部分中表现出α2(I)和al(XII)探针的共表达。这项研究表明，多西环素和 CMT-1 可以减轻或减少牙周感染对 I 型和 XII 型胶原蛋白 mRNA 表达的抑制作用。这些结果表明，强力霉素和一种非抗菌四环素（化学修饰的四环素-1）可以通过逆转牙周感染对胶原蛋白合成的抑制作用来减少牙周破坏。
TISSUE REMODELING IS A DYNAMIC State in which a balance is achieved between the proteolytic breakdown and synthesis of the extracellular matrix. Type I collagen is a major component of the gingival connective tissue (GCT) and the periodontal ligament (PDL) throughout development, while type XII collagen has been found in the mature forms of these tissues. The purpose of this study was to investigate the effects of periodontitis on the expression of type I and XII collagen and subsequently to investigate the effects of doxycycline (DOXY) and chemically modified nonantimicrobial tetracycline (CMT-1) on the expression of these molecules in this model. Adult barrier-raised male Sprague-Dawley rats were inoculated with Porphyromonas gingivalis obtained from humans to create the experimental periodontitis. The animals with the P. gingivalis-induced periodontitis were then split into the following groups: Group A served as infected untreated controls (PGI group); group B was treated with doxycycline (DOXY group); and group C was treated with chemically modified tetracycline-l (CMT-1 group). Group D contained uninfected animals that served as uninfected controls (NIC group). The expression of type I and XII collagen mRNAs was examined by in situ hybridization in each group, with the co-expression of these molecules representing mature and functional gingival connective tissue. In the NIC group, cells hybridized with digoxygenine-labeled cDNA probes encoding rat alpha 2(I) or alpha 1(XII) collagens were found distributed uniformly throughout the periodontal connective tissue. The PGI group showed little hybridization in the areas of infection, while both the DOXY and CMT-1 groups showed co-expression of the alpha 2(I) and al(XII) probes in the GCT and coronal part of the PDL. This study demonstrates that doxycycline and CMT-1 moderate or reduce the inhibitory effects of periodontal infection on the expression of type I and type XII collagen mRNAs. These results suggest that doxycycline and a form of non-antimicrobial tetracycline, chemically modified tetracycline-l, can reduce periodontal destruction by reversing the inhibitory effect of periodontal infection on collagen synthesis.