Determination of vitamin B12 using a chemiluminescence flow system
Determination of vitamin B12 using a chemiluminescence flow system
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DOI：
10.1134/s1061934808110105
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发表时间：
2008-10
影响因子：
1.1
通讯作者：
N. Akbay;E. Gök
中科院分区：
文献类型：
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作者：
N. Akbay;E. Gök
提出了一种用流动注射化学发光法测定维生素B12的方法。它是基于维生素B_（12）中的钴（II）对碱性介质中鲁米诺与过氧化氢的化学发光反应的催化作用。维生素B_（12）的浓度在8.68-86.9 ng/mL范围内与化学发光强度的增量呈线性关系（r ~ 2 = 0.9984），检出限（3σ）为0.89 ng/mL。在3.0 mL/min的流速下，在10 s内获得CL响应，相对标准偏差（RSD）小于2.5%（n= 6）。该方法已成功地应用于注射剂中维生素B12的测定。
A method to determine vitamin B12by measuring the chemiluminescence (CL) intensities using a flow injection (FI) system has been proposed. It is based on the catalytic effect of cobalt(II) in vitamin B12on the CL reaction between luminol and hydrogen peroxide in a basic medium. The increment of the CL intensity is proportional to the concentration of vitamin B12in the range 8.68–86.9 ng/mL (r2= 0.9984) with a detection limit (3σ) of 0.89 ng/mL. The CL response is obtained in 10 s at a flow rate of 3.0 mL/min with a relative standard deviation (RSD) of less than 2.5% (n= 6). The method has been successfully applied to the determination of vitamin B12in pharmaceutical injections.