Highly sensitive and selective dual-emission ratiometric fluorescence detection of dopamine based on carbon dots-gold nanoclusters hybrid
Highly sensitive and selective dual-emission ratiometric fluorescence detection of dopamine based on carbon dots-gold nanoclusters hybrid
复制标题
基于碳点-金纳米团簇杂化的多巴胺高灵敏选择性双发射比率荧光检测
DOI：
10.1016/j.snb.2018.03.080
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发表时间：
2018-07-15
影响因子：
8.4
通讯作者：
Liang, Guo-Xi
中科院分区：
文献类型：
--
作者：
He, Yu-Sheng;Pan, Chang-Gang;Liang, Guo-Xi
本工作制备了一种新型的基于荧光共振能量转移（FRET）的比率荧光探针--碳点-金纳米团簇杂化物（CDots-AuNCs），用于多巴胺（DA）的选择性、灵敏性和可靠性检测。该FRET探针由两个荧光团组成，其中碳量子点（CDots）作为能量供体，金纳米团簇（AuNC）作为受体，在380 nm的单一激发波长下在420 nm和610 nm处具有双发射峰。加入DA后，610 nm处荧光猝灭，420 nm处蓝色荧光恢复。通过监测在420和610 nm处的比率荧光强度的变化，DA可以在5至180 nM的范围内检测，检测限约为2.9 nM。最后，该传感方法成功地应用于血清样品中DA的测定，回收率在95%-105%之间。(C)2018 Elsevier B. V.版权所有。
In this work, a novel fluorescence resonance energy transfer (FRET)-based ratiometric fluorescent probe, carbon dots-gold nanoclusters hybrid (CDots-AuNCs), was fabricated for selective, sensitive and reliable sensing of dopamine (DA). This FRET probe is comprised of a two-fluorophore, where carbon dots (CDots) serve as the energy donor and gold nanoclusters (AuNCs) as the acceptor, with dual emission peaks at 420 nm and 610 nm under a single excitation wavelength of 380 nm. The addition of DA to this probe solution resulted in the fluorescence at 610 nm quenching, while the blue fluorescence at 420 nm recovering. By monitoring the change of ratiometric fluorescent intensity at 420 and 610 nm, the DA could be detected with the range from 5 to 180 nM and a limit of detection around 2.9 nM. Finally, the developed sensing method was successfully applied to DA determination in serum samples with satisfactory recoveries in the range of 95%-105%. (C) 2018 Elsevier B.V. All rights reserved.