SIGMA-FACTORS FROM ESCHERICHIA-COLI, BACILLUS-SUBTILIS, PHAGE-SP01, AND PHAGE-T4 ARE HOMOLOGOUS PROTEINS
SIGMA-FACTORS FROM ESCHERICHIA-COLI, BACILLUS-SUBTILIS, PHAGE-SP01, AND PHAGE-T4 ARE HOMOLOGOUS PROTEINS
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DOI：
10.1093/nar/14.16.6745
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发表时间：
1986-08-26
影响因子：
14.9
通讯作者：
BURGESS, RR
中科院分区：
文献类型：
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作者：
GRIBSKOV, M;BURGESS, RR
我们通过点阵比较和序列比对的统计分析表明，7个测序的sigma因子，E.colisigma-70和sigma-32，B.subtilissigma-43和sigma-29，噬菌体SP01基因产物28和34i，以及噬菌体T4基因产物55，组成了一个同源的蛋白质家族。Sigma-70、Sigma-32和Sigma-43都有两个拷贝的序列，类似于CRP中看到的螺旋-转弯-螺旋DNA结合基序，以及lambda阻遏物和CRO蛋白。B.subtilissigma-29、SP01 gp28和SP01 gp34至少有一个拷贝与该序列相似。我们提出了第二个序列，保守在所有七种蛋白质中的S核心核糖核酸聚合酶结合部位。仅存在于sigma-70和sigma-43中的第三个区域也可能参与与核心的相互作用。现有的突变证据支持我们的西格玛因子结构模型。
We show, using dot matrix comparisons and statistical analysis of sequence alignments, that seven sequenced sigma factors,E.colisigma-70 and sigma-32,B.subtilissigma-43 and sigma-29, phage SP01 gene products 28 and 34i and phage T4 gene product 55, comprise a homologous family of proteins. Sigma-70, sigma-32, and sigma-43 each have two copies of a sequence similar to the helix-turn-helix DNA binding motif seen in CRP, and lambda repressor and cro proteins.B.subtilissigma-29, SP01 gp28, and SP01 gp34 have at least one copy similar to this sequence. We propose that a second sequence, conserved in all seven proteins i s the core RNA polymerase binding site. A third region, present only in sigma-70 and sigma-43, may also be involved in interaction with core. Available mutational evidence supports our model for sigma factor structure.