Molecular cloning and characterization of a transferrin cDNA from the white-spotted flower chafer, Protaetia brevitarsis
Molecular cloning and characterization of a transferrin cDNA from the white-spotted flower chafer, Protaetia brevitarsis
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DOI：
10.1080/10425170701461854
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发表时间：
2008-04-01
期刊：
影响因子：
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通讯作者：
Jin, Byung Rae
中科院分区：
文献类型：
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作者：
Kim, Bo Yeon;Lee, Kwang Sik;Jin, Byung Rae
通过筛选短螺旋藻（Protaetia brevitarsis） cDNA文库，克隆出一个与昆虫转铁蛋白基因高度同源的全长cDNA克隆。该基因全长2338 bp，开放阅读框（ORF） 2163 bp，编码721个氨基酸残基的预测肽。与已知的蟑螂、白蚁和甲虫转铁蛋白一样，PbTf似乎在N端和c端叶中都含有铁结合位点的残基。PbTf cDNA的氨基酸序列在结构上与天牛转铁蛋白最接近（68%的蛋白序列同源性）。Northern blot分析显示，PbTf表现出脂肪体特异性表达，并在损伤、细菌或真菌感染和铁超载的情况下上调，提示PbTf在防御和应激反应中发挥功能作用。
A full-length cDNA clone with high homology to insect transferrin genes was cloned by screening a Protaetia brevitarsis cDNA library. This gene (PbTf) had a total length of 2338 bp with an open reading frame (ORF) of 2163 bp, and encoded a predicted peptide of 721 amino acid residues. Like known cockroach, termite, and beetle transferrins, PbTf appears to have residues comprising iron-binding sites in both N- and C-terminal lobes. The deduced amino acid sequence of the PbTf cDNA was closest in structure to the beetle Apriona germari transferrin (68% protein sequence identity). Northern blot analysis revealed that PbTf exhibited fat body-specific expression and was upregulated by wounding, bacterial or fungal infection and iron overload, suggesting a functional role for PbTf in defense and stress responses.