Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model
Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model
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DOI：
10.1099/jmm.0.46660-0
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发表时间：
2006-11-01
影响因子：
3
通讯作者：
Kamiya, Shigeru
中科院分区：
文献类型：
--
作者：
Osaki, Takako;Hanawa, Tomoko;Kamiya, Shigeru
幽门螺杆菌与人类和一些实验动物的胃部疾病有关，并具有luxS/2型自诱导物（AI-2）系统。研究了一种特异性luxS突变对H. pylori检查。在0（.）3%琼脂培养基上，H. pylori HPKY 08（luxS：：cat）的表达显著低于野生型H. pylori TK 1402。补充luxS的菌株HPKY 21表现出与H. pylori TK 1402。结果表明，luxS/AI-2系统在H.幽门运动在蒙古长爪沙鼠模型中，luxS突变体相对于野生型亲本菌株TK 1402表现出降低的感染率。口服接种后3个月，H. pylori在luxS（-）mux感染的沙鼠中的半定量实时逆转录PCR（qRT-PCR）检测结果明显高于TK 1402感染的沙鼠。胃炎症和H抗体滴度增加。pylori感染的沙鼠。
Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (AI-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0(.)3% agar medium, motility of H. pylori HPKY08 (luxS: : cat) was significantly lower than that of wild-type H. pylori TK1402. The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/AI-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS(-) mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.