circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty.
circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty.
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DOI：
10.7150/ijms.48014
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发表时间：
2021
影响因子：
3.6
通讯作者：
Guo L
中科院分区：
文献类型：
--
作者：
Ni S;Jiang T;Hao S;Luo P;Wang P;Almatari Y;Wang Y;Zhang Z;Guo L
越来越多的证据表明，环状RNA（circRNA）在某些疾病的发病机制中发挥着重要作用。然而，circRNA在全髋关节置换术（THA）后无菌性松动中的作用仍然很大程度上未知。本研究旨在探索无菌性松动中差异表达的circRNA（DECircRNA），阐明circRNA在无菌性松动中的复杂调控机制。通过RNA测序（RNA-seq）分析鉴定DECircRNA。采用逆转录-定量聚合酶链反应（RT-qPCR）证实这些DECircRNA。通过竞争性内源性RNA（ceRNA）分析确定了circRNA在无菌性松动组织中的潜在功能。通过基因肿瘤学（GO）和京都基因和基因组百科全书（KEGG）对DECircRNA的靶mRNA和宿主基因进行富集分析。从RNA-seq结果获得257个DECircRNA。在RT-qPCR确证后，选择6种circRNA（hsa_circ_0007482、hsa_circ_0005232、hsa_circ_0000994、hsa_circ_0000690、hsa_circ_0058092和hsa_circ_0004496）用于进一步分析。通过circRNA-miRNA和miRNA-mRNA预测，共鉴定出6个circRNA、138个miRNA和1667个mRNAs。建立了circRNA-miRNA-mRNA网络。GO和KEGG富集分析结果提示，circRNA与某些生物学功能和无菌性松动的发生途径有关。我们对circRNA-miRNA-mRNA网络的研究揭示了THA术后无菌性松动的新的发病机制和治疗策略。
Increasing evidence has demonstrated that circular RNA (circRNA) exerts important function in the pathogenesis of some diseases. While, the contributions of circRNAs to aseptic loosening after total hip arthroplasty (THA) remain largely unknown. Our research is to explore the differentially expressed circRNAs (DEcircRNAs) and elucidate complex regulated mechanism of circRNAs in aseptic loosening. The DEcircRNAs were identified by RNA sequencing (RNA-seq) analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was adopted to corroborate these DEcircRNAs. The potential function of circRNAs in aseptic loosening tissue was identified by competing endogenous RNA (ceRNA) analysis. Enrichment analysis was performed for target mRNAs and host genes of the DEcircRNAs by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 257 DEcircRNAs were obtained from RNA-seq results. Following the RT-qPCR corroboration, 6 circRNAs (hsa_circ_0007482, hsa_circ_0005232, hsa_circ_0000994, hsa_circ_0000690, hsa_circ_0058092 and hsa_circ_0004496) were selected for further analysis. By circRNA-miRNA and miRNA-mRNA prediction, 6 circRNAs, 138 miRNAs and 1667 mRNAs were identified. Then, circRNA-miRNA-mRNA network was established. The result of GO and KEGG enrichment analysis suggested that the circRNAs were related with some biological functions and pathways of aseptic loosening. A novel pathogenesis and treatment strategy about aseptic loosening after THA was revealed from our study of circRNA-miRNA-mRNA network.
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影响因子：
13.3
作者：
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通讯作者：
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64.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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