The immunostimulatory effects and chemical characteristics of heated honey
The immunostimulatory effects and chemical characteristics of heated honey
复制标题
加热蜂蜜的免疫刺激作用和化学特性
DOI：
10.1016/j.jep.2018.09.019
复制
发表时间：
2019
影响因子：
5.4
通讯作者：
Makino Toshiaki
中科院分区：
文献类型：
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作者：
Ota Misato;Ishiuchi Kan’ichiro;Xu Xin;Minami Masaaki;Nagachi Yasutaka;Yagi-Utsumi Maho;Tabuchi Yoshiaki;Cai Shao-Qing;Makino Toshiaki
民族药理学相关性在传统中药（TCM）中，蜂蜜被用作草药加热加工中的添加剂，以增强其免疫刺激活性。本研究目的我们研究了加热蜂蜜的体外和体内免疫刺激活性。材料和方法对于体外研究，我们比较了不同加热条件下蜂蜜对人体的诱导作用的差异。 培养的肠上皮细胞分泌粒细胞集落刺激因子（G-CSF），并研究其活性成分。在体内研究中，我们对感染化脓性链球菌的小鼠进行了存活试验，无论是否口服加热的蜂蜜。结果我们发现，加热蜂蜜会诱导培养的肠细胞出现G-CSF分泌物，并且这种分泌物的出现取决于加热温度和时间。蜂蜜不加热时无G-CSF分泌物出现。感染化脓性链球菌的小鼠被喂食加热的蜂蜜后，其存活时间得以延长。加热蜂蜜中的活性成分是一种分子量约为730kDa的高分子化合物。当该化合物水解时，生成半乳糖、葡萄糖、鼠李糖、α-呋喃核糖、β-呋喃核糖1,5':1'、5-二酐和5-羟甲基糠醛。结论加热的蜂蜜在体外和体内均具有免疫刺激活性。这些结果支持了中医理论的科学证据。
Ethnopharmacological relevanceIn traditional Chinese medicine (TCM), honey has been used as an additive in the heat-processing of herbal medicines to enhance their immunostimulatory activities.Aim of the studyWe investigated the immunostimulatory activity of heated honeyin vitroandin vivo.Materials and methodsFor thein vitrostudy, we compared the differences among the inducible effects of honey subjected to various heating conditions on granulocyte colony-stimulating factor (G-CSF) secretion from the cultured enterocytes and investigated the active ingredient. For thein vivostudy, we conducted a survival test of mice infected byStreptococcus pyogeneswith and without oral administration of heated honey.ResultsWe found that heating the honey induced the appearance of G-CSF secretions from the cultured enterocytes, and that this appearance depended on the heating temperature and time. No G-CSF secretions appeared when honey was not heated. Mice infected withStreptococcus pyogenesthat were fed heated honey revealed prolonged survival. The active ingredient in heated honey was a high-molecular compound with about 730 kDa. When this compound was hydrolyzed, galactose, glucose, rhamnose,α-ribofuranoseβ-ribofuranose 1,5′:1′,5-dianhydride, and 5-hydroxymethylfurfural were generated.ConclusionsHeated honey reveals immunostimulatory activity bothin vitroandin vivo. These results support the scientific evidences of the TCM theory.