Anatomic analysis of blood vessels in germinal matrix, cerebral cortex, and white matter in developing infants
Anatomic analysis of blood vessels in germinal matrix, cerebral cortex, and white matter in developing infants
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DOI：
10.1203/01.pdr.0000130472.30874.ff
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发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Nedergaard, M
中科院分区：
文献类型：
--
作者：
Ballabh, P;Braun, A;Nedergaard, M
生发基质(GM)位于丘脑纹状体沟厚厚的室管膜下细胞层，是早产儿脑出血的主要部位。比较GM、脑灰质和脑白质的血管形态有助于了解GM出血和脑室周围白质软化的发病机制。本研究的目的是确定巨噬细胞、灰质和白质中血管的形态，并检测这些血管形态随胎龄的成熟变化。我们测量了17例胎龄16~40周的胎儿和早产儿和2例成人身体脑标本中GM、灰质和白质冠状切片的血管密度、血管面积百分比、平均表面积、长度、宽度、周长、半径和血管形状。我们使用抗层粘连蛋白初级抗体进行免疫组织化学染色，共聚焦显微镜获取图像，并使用Metamorph版本6.1进行分析。在GM、灰质和白质中，血管密度和血管面积百分比随着胎龄的增加而增加(p<；各0.001)。血管密度和血管面积百分比在GM中最大，其次是灰质，然后是白质，在所有胎龄类别中(P<；所有比较为0.001)。与灰质和白质相比，GM的血管增多可能在GM出血中起作用，而相对较低的白质血管可能增加早产儿发生脑室周围白质软化的倾向。
The germinal matrix (GM) located in the thick subependymal cell layer of the thalamostriate groove is a major site of cerebral hemorrhage in premature infants. Comparing the morphology of vasculature among GM, gray and white matter of the brain may help in understanding the pathogenesis of GM hemorrhage and also of periventricular leukomalacia. The objective of the present study was to determine the morphology of blood vessels in the GM, gray matter, and white matter and to examine maturational changes in the morphology of these vessels as a function of gestational age. We measured vessel density, percentage of blood vessel area, mean surface area, length, breadth, perimeter, radius, and shape of blood vessels in coronal sections of the GM, gray matter, and white matter in postmortem human brain samples for 17 fetuses and premature infants of gestational age 16-40 wk and 2 adults. We performed immunohistochemical staining using anti-laminin primary antibody, confocal microscopy to acquire images, and analysis using Metamorph version 6.1. Vessel density and the percentage of blood vessel area increased as a function of gestational age in the GM, gray matter, and white matter (p < 0.001 each). The blood vessel density and the percentage of blood vessel area were largest in the GM followed by gray matter and then white matter in all of the gestational age categories (p < 0.001 for all comparisons). Increased vascularity of the GM compared with gray and white matter may play a role in GM hemorrhage, whereas a relatively low vascularity of white matter may increase the propensity for the occurrence of periventricular leukomalacia in premature infants.