Protective Effects of Enzyme-assistant Extracts of Sargassum fulvellum against AAPH-induced Oxidative Stress in vitro in Vero Cells
Protective Effects of Enzyme-assistant Extracts of Sargassum fulvellum against AAPH-induced Oxidative Stress in vitro in Vero Cells
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DOI：
10.17642/jcc.23.2.7
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
WANGLEI
中科院分区：
文献类型：
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作者：
Jeon, You-Jin;Oh, Jae-Young;WANGLEI
采用酶法辅助提取法提取了褐皮马尾藻的粗提物，并对提取物的主要成分进行了测定。酶辅助提取法的提取率比水提取法提高了4.67 ~ 9.67%。使用电子自旋共振（ESR）光谱法评价这些浸提液的自由基清除活性，并通过测量Vero细胞活力评价这些浸提液的细胞毒性。Alcalase提取物（SFA-2）、Celluclast提取物（SFC）、Kojizyme提取物（SFK）和Neutrase提取物（SFN）在Vero细胞中显示出较强的自由基清除活性，并且与其他提取物相比无毒性。评价了SFA-2、SFC、SFK和SFN对AAPH诱导的Vero细胞氧化应激的保护作用。在AAPH诱导的Vero细胞中，25、50和100 μg/mL浓度的SFC表现出最强的保护作用，可清除35.33%、38.75%和47.69%的细胞内活性氧自由基（ROS），并提高5.89%、8.36%和12.13%的细胞活力。因此，本研究表明，酶法辅助提取是一种提取藻类抗氧化剂的有效方法，SFC具有较强的抗氧化活性，可作为医药和化妆品工业的理想原料。
In the present study, Sargassum fulvellum was extracted by enzyme-assistant extraction and the proximate compositions of these extracts were determined. The extraction yields of enzyme-assistant extraction were improved 4.67 to 9.67% compared to water extraction. The free radical scavenging activity of these extracts was evaluated using Electron Spin Resonance (ESR) spectroscopy and the cytotoxicity of these extracts was evaluated by measuring the viability of Vero cells. The Alcalase extract (SFA-2), Celluclast extract (SFC), Kojizyme extract (SFK), and Neutrase extract (SFN) were shown stronger free radical scavenging activity and non-toxicity in Vero cells compared with other extracts. The protective effects of SFA-2, SFC, SFK, and SFN against AAPH-induced oxidative stress in Vero cells were evaluated. SFC showed the strongest protective effect that indicated in scavenging 35.33, 38.75, and 47.69% intracellular reactive oxygen species (ROS) and improving 5.89, 8.36, and 12.13% viability at the concentration of 25, 50, and 100 μg/mL in AAPH-induced Vero cells. Therefore, this study demonstrated that enzyme-assistant extraction is an effective extraction method to extract antioxidant from algae, and SFC possesses strong antioxidant activity may be used as an ideal ingredient in pharmaceutical and cosmeceutical industries.