Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake
Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake
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参与硫代硫酸盐吸收的 YeeE/YedE 家族蛋白的晶体结构
DOI：
10.1126/sciadv.aba7637
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发表时间：
2020
期刊：
影响因子：
13.6
通讯作者：
Tsukazaki Tomoya
中科院分区：
文献类型：
--
作者：
Tanaka Yoshiki;Yoshikaie Kunihito;Takeuchi Azusa;Ichikawa Muneyoshi;Mori Tomoyuki;Uchino Sayaka;Sugano Yasunori;Hakoshima Toshio;Takagi Hiroshi;Nonaka Gen;Tsukazaki Tomoya
我们已经证明了细菌膜蛋白Yeee E介导硫代硫酸盐摄取。硫代硫酸盐作为全球生物硫循环中的无机硫源，用于细菌中半胱氨酸的合成。2.5 Å分辨率的YeeE晶体结构揭示了前所未有的沙漏状结构，带正电的外凹侧具有硫代硫酸盐。YeeE由环状结构和13个螺旋组成，其中9个为跨膜α螺旋，大部分为分子内假222对称性。四个特征环被埋向Yeee E的中心，并形成由九个螺旋包围的中心区域。额外的电子密度图和连续的分子动力学模拟表明，硫代硫酸盐可以暂时保持在几个位置在拟议的途径。我们提出了一个合理的机制，硫代硫酸盐摄取通过三个重要的保守半胱氨酸残基的环路沿着的途径。
We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-Å resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane α helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway.