Microfluidic patterning of cells in extracellular matrix biopolymers: Effects of channel size, cell type, and matrix composition on pattern integrity
Microfluidic patterning of cells in extracellular matrix biopolymers: Effects of channel size, cell type, and matrix composition on pattern integrity
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DOI：
10.1089/107632703764664729
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发表时间：
2003-04-01
影响因子：
--
通讯作者：
Desai, TA
中科院分区：
文献类型：
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作者：
Tan, W;Desai, TA
细胞在细胞外基质内的组织对于促进体内适当的细胞相互作用和组织功能至关重要。通过表面上的微流体图案化设计和创建生物相关细胞排列的能力为组织工程和仿生学提供了新的能力。本文的目的是描述使用三维生物聚合物基质的微流体图案化技术，以提高细胞图案的完整性，并提供对细胞微环境的微尺度控制。结果表明，在细胞微流控图案化中掺入细胞外基质生物聚合物在体外数天后比没有基质组分的图案化产生更稳定的粘附人内皮细胞图案。这对开展长期的体外生物学实验和组织工程研究具有重要意义。此外，图案化的生物聚合物基质中的化学成分，如胶原蛋白、壳聚糖和纤连蛋白，影响基质随时间控制细胞迁移和图案稳定性的能力。因此，细胞外基质生物聚合物中的细胞的微流体图案化显示出可用于在明确定义的三维几何形状中图案化多种细胞类型。
The organization of cells within an extracellular matrix is critical to promote appropriate cellular interactions and tissue function in vivo. The ability to design and create biologically relevant cellular arrangements via microfluidic patterning on surfaces provides new capabilities for tissue engineering and biomimetics. The purpose of this article is to describe techniques using microfluidic patterning of three-dimensional biopolymer matrices to improve cellular pattern integrity and to provide microscale control over cellular microenvironments. Results demonstrated that the incorporation of extracellular matrix biopolymers in cell microfluidic patterning results in a more stable pattern of adherent human endothelial cells than patterning without matrix components after several days in vitro. This may be important for carrying out long-term biological experiments and tissue engineering in vitro. Moreover, chemical components in the patterned biopolymer matrices, such as collagen, chitosan, and fibronectin, influenced the ability of the matrices to control cell migration and pattern stability over time. Thus, microfluidic patterning of cells in extracellular matrix biopolymers was shown to be useful in patterning multiple cell types in well-defined three-dimensional geometries.