Ultrahigh-resolution full-field optical coherence tomography
Ultrahigh-resolution full-field optical coherence tomography
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DOI：
10.1364/ao.43.002874
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发表时间：
2004-05-10
期刊：
影响因子：
1.9
通讯作者：
Boccara, C
中科院分区：
文献类型：
--
作者：
Dubois, A;Grieve, K;Boccara, C
我们开发了一种用于生物介质超高分辨率全场光学相干层析成像的白光干涉显微镜。实验装置是基于林尼克型干涉仪由卤钨灯照明。通过高速CCD相机记录的干涉图像的组合来计算正面断层图像。空间分辨率为1.8妈妈x0.9妈妈（横向x轴向）是由于实现了极短的相干长度的源，在干涉仪臂色散失配的补偿，并使用相对较高的数值孔径显微镜物镜。在每幅图像4 s的采集时间内获得了90 dB的散粒噪声限制检测灵敏度。植物，动物和人体组织的亚细胞水平的图像。(C)2004年美国光学学会。
We have developed a white-light interference microscope for ultrahigh-resolution full-field optical coherence tomography of biological media. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp. En face tomographic images are calculated by a combination of interferometric images recorded by a high-speed CCD camera. Spatial resolution of 1.8 mum x 0.9 mum (transverse x axial) is achieved owing to the extremely short coherence length of the source, the compensation of dispersion mismatch in the interferometer arms, and the use of relatively high-numerical-aperture microscope objectives. A shot-noise-limited detection sensitivity of 90 dB is obtained in an acquisition time per image of 4 s. Subeellular-level images of plant, animal, and human tissues are presented. (C) 2004 Optical Society of America.