Measurement of purine release with microelectrode biosensors
Measurement of purine release with microelectrode biosensors
复制标题
DOI：
10.1007/s11302-011-9273-4
复制
发表时间：
2012-02-01
影响因子：
3.5
通讯作者：
Frenguelli, Bruno G.
中科院分区：
文献类型：
--
作者：
Dale, Nicholas;Frenguelli, Bruno G.
嘌呤能信号传导在细胞外ATP和腺苷的多种释放机制和产生途径上不同于传统的神经传递范式。这些嘌呤能药物的直接实时测量对于理解该信号系统在许多不同背景下的功能作用具有重要价值。在这里，我们回顾了测量嘌呤释放的方法，介绍了ATP和腺苷的微电极生物传感器的概念，并解释了这些已被用来提供新的机械洞察呼吸化学感受，突触生理学，眼睛发育和嘌呤抢救。最后，我们考虑嘌呤释放与病理条件的关联，并研究嘌呤生物传感器有朝一日可能成为临床诊断工具箱的标准部分的可能性。
Purinergic signalling departs from traditional paradigms of neurotransmission in the variety of release mechanisms and routes of production of extracellular ATP and adenosine. Direct real-time measurements of these purinergic agents have been of great value in understanding the functional roles of this signalling system in a number of diverse contexts. Here, we review the methods for measuring purine release, introduce the concept of microelectrode biosensors for ATP and adenosine and explain how these have been used to provide new mechanistic insight in respiratory chemoreception, synaptic physiology, eye development and purine salvage. We finish by considering the association of purine release with pathological conditions and examine the possibilities that biosensors for purines may one day be a standard part of the clinical diagnostic tool chest.