STAT 5 activators can replace the requirement of FBS in the adipogenesis of 3T3-L1 cells.
STAT 5 activators can replace the requirement of FBS in the adipogenesis of 3T3-L1 cells.
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DOI：
10.1016/j.bbrc.2004.09.053
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发表时间：
2004-11
影响因子：
3.1
通讯作者：
William C. Stewart;J. Baugh;Z. Floyd;J. Stephens
中科院分区：
文献类型：
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作者：
William C. Stewart;J. Baugh;Z. Floyd;J. Stephens
3T3-L1细胞分化成具有天然脂肪细胞的许多特性的脂肪细胞，包括：大量脂质积累、胰岛素敏感性和分泌内分泌激素的能力。使用这些细胞的实质性费用是胎牛血清（FBS），其是有效脂肪形成的关键组分。我们最近对STAT 5蛋白的研究表明，这些转录因子在分化开始后立即被磷酸化并易位到细胞核。其他几个实验室的研究也表明STAT 5蛋白可以具有促脂肪形成的特性。生长激素（GH）和催乳素（PRL）都是STAT 5A和STAT 5B蛋白的有效激活剂。由于FBS具有高浓度的GH;我们检查了GH替代FBS作为3T3-L1细胞的分化混合物的组分的能力。我们的研究表明，FBS是不需要的3T3-L1细胞的脂肪形成，如果GH或PRL添加到分化鸡尾酒。通过油红O染色和脂肪细胞标志基因的表达来判断脂肪生成。因此，我们已经开发了一种基本上更便宜的方法来分化3T3-L1细胞，而不需要FBS、噻唑烷二酮或昂贵的细胞因子。
The 3T3-L1 cells differentiate into fat cells that have many properties of native adipocytes including: substantial lipid accumulation, insulin sensitivity, and the ability to secrete endocrine hormones. A substantial expense in using these cells is fetal bovine serum (FBS), a critical component of efficient adipogenesis. Our recent studies on STAT 5 proteins have revealed that these transcription factors are phosphorylated and translocate to the nucleus immediately after the initiation of differentiation. Studies by several other laboratories also suggest that STAT 5 proteins can have pro-adipogenic properties. Growth hormone (GH) and prolactin (PRL) are both potent activators of STAT 5A and STAT 5B proteins. Since, FBS has high concentrations of GH; we examined the ability of GH to replace FBS as a component of the differentiation cocktail for 3T3-L1 cells. Our studies revealed that FBS was not required for the adipogenesis of 3T3-L1 cells if GH or PRL was added to the differentiation cocktail. Adipogenesis was judged by Oil Red O staining and expression of adipocyte marker genes. Hence, we have developed a substantially less expensive method for differentiating 3T3-L1 cells without FBS, thiazolidinediones, or expensive cytokines.