Transient state kinetics tutorial using the kinetics simulation program, KINSIM.
Transient state kinetics tutorial using the kinetics simulation program, KINSIM.
复制标题
使用动力学模拟程序 KINSIM 的瞬态动力学教程。
DOI：
10.1016/s0006-3495(94)80598-3
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发表时间：
1994
影响因子：
3.4
通讯作者：
Pollard,TD
中科院分区：
文献类型：
--
作者：
Wachsstock,DH;Pollard,TD
这篇文章介绍了一个关于暂态动力学的计算机教程。该教程使用的是我们的Macintosh版本的计算机程序KINSIM，它可以计算反应的时间进程。KINSIM也适用于其他流行的电脑。该程序甚至允许那些在数学上不倾向于评估任何反应机理中中间体之间的转变的速率常数的研究人员。这些速率常数是了解生化过程如何在分子水平上工作的基本见解之一。这种方法不仅适用于酶反应，也适用于生物物理学家、细胞生物学家和分子生物学家感兴趣的任何其他类型的过程，其中浓度随时间变化。原则上，同样的方法也可以用来描述生态学和进化论中依赖时间的大规模过程。完成教程需要学生6-10小时。这项投资的回报是对化学动力学原理的深入理解，并熟悉作为解决实验室日常问题的动力学模拟工具。
This article provides an introduction to a computer tutorial on transient state kinetics. The tutorial uses our Macintosh version of the computer program, KINSIM, that calculates the time course of reactions. KINSIM is also available for other popular computers. This program allows even those investigators not mathematically inclined to evaluate the rate constants for the transitions between the intermediates in any reaction mechanism. These rate constants are one of the insights that are essential for understanding how biochemical processes work at the molecular level. The approach is applicable not only to enzyme reactions but also to any other type of process of interest to biophysicists, cell biologists, and molecular biologists in which concentrations change with time. In principle, the same methods could be used to characterize time-dependent, large-scale processes in ecology and evolution. Completion of the tutorial takes students 6–10 h. This investment is rewarded by a deep understanding of the principles of chemical kinetics and familiarity with the tools of kinetics simulation as an approach to solve everyday problems in the laboratory.