Rhinovirus transmission within families with children:: Incidence of symptomatic and asymptomatic infections
Rhinovirus transmission within families with children:: Incidence of symptomatic and asymptomatic infections
复制标题
DOI：
10.1086/525542
复制
发表时间：
2008-02-01
影响因子：
6.4
通讯作者：
Hyypia, Timo
中科院分区：
文献类型：
--
作者：
Peltola, Ville;Waris, Matti;Hyypia, Timo
背景鼻病毒是呼吸道感染最常见的病原体，但其在家庭中的传播尚缺乏敏感和特异的分子检测方法。因任何感染住院的儿童都接受了鼻病毒筛查。8个家庭的鼻病毒阳性指数儿童和16个家庭的鼻病毒阴性指数儿童进行了3周的疾病症状监测，鼻拭子样本中鼻病毒的存在和数量进行了定量逆转录-聚合酶链反应。通过解链温度和部分序列分析进一步鉴定鼻病毒。鼻病毒阳性指数患者的17名同胞中鼻病毒感染率为1.00例/人，14名父母中鼻病毒感染率为0.50例/人;鼻病毒阴性指数患者的24名同胞中鼻病毒感染率为0.54例/人，30名父母中鼻病毒感染率为0.23例/人。症状性感染与年龄相关，
Background. Rhinoviruses are the most common cause of respiratory tract infections, but the transmission in families has not been studied using sensitive and specific molecular detection methods.Methods. Children hospitalized for any infection were screened for rhinoviruses. Eight families with a rhinovirus-positive index child and 16 families with a rhinovirus-negative index child were monitored for 3 weeks for disease symptoms, and the presence and quantity of rhinoviruses in nasal swab samples were determined by quantitative reverse transcription-polymerase chain reaction. Rhinoviruses were further identified by melting temperature and partial sequence analysis.Results. The rates of rhinovirus infection were 1.00 cases per person among the 17 siblings and 0.50 cases per person among the 14 parents of rhinovirus-positive index patients; the rates were 0.54 cases per person among the 24 siblings and 0.23 cases per person among the 30 parents of rhinovirus-negative index patients. Symptomatic infections were associated with an age of