An improved red blood cell additive solution maintains 2,3-diphosphoglycerate and adenosine triphosphate levels by an enhancing effect on phosphofructokinase activity during cold storage
An improved red blood cell additive solution maintains 2,3-diphosphoglycerate and adenosine triphosphate levels by an enhancing effect on phosphofructokinase activity during cold storage
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DOI：
10.1111/j.1537-2995.2010.02700.x
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发表时间：
2010-11-01
期刊：
影响因子：
2.9
通讯作者：
Verhoeven, Arthur J.
中科院分区：
文献类型：
--
作者：
Burger, Patrick;Korsten, Herbert;Verhoeven, Arthur J.
背景：目前用于红细胞（rbc）的添加剂溶液（ASs）在冷藏期间不能维持2,3-二磷酸甘油酸（DPG）和三磷酸腺苷（ATP）的恒定水平。我们之前已经表明，使用一种新的称为磷酸腺苷-葡萄糖-鸟苷-葡萄糖-甘油三醇（PAGGGM）的添加剂溶液，2,3-二磷酸甘油三酯和ATP可以在整个储存过程中维持35天。在本研究中，我们更详细地研究了PAGGGM影响红细胞储存的机制。通过双红细胞分离装置（白细胞诱导），可以直接比较当前的AS盐-腺-葡萄糖-甘露醇（SAGM）和实验溶液PAGGGM在冷藏过程中的一些体外特性。结果：与我们之前对单个红细胞的研究结果一致，PAGGGM在35天的冷库中保持了2,3- dpg和ATP的水平，并且在冷库的前21天，PAGGGM单元的葡萄糖消耗和乳酸产量较高，果糖-1,6-二磷酸和磷酸二羟丙酮的水平也在前21天升高。这些结果表明，相对于SAGM单位，PAGGGM单位的磷酸果糖激酶（PFK）活性在21天后可能会增强，PAGGGM单位的PFK活性也会降低，但这些单位中足够的代谢储备可以防止2,3- dpg和ATP的消耗
BACKGROUND: Current additive solutions (ASs) for red blood cells (RBCs) do not maintain constant 2,3-diphosphoglycerate (DPG) and adenosine triphosphate (ATP) levels during cold storage We have previously shown that with a new AS called phosphate-adenine-glucose-guanosine-gluconate-mannitol (PAGGGM), both 2,3-DPG and ATP could be maintained throughout storage for 35 daysSTUDY DESIGN AND METHODS: In this study, the mechanism underlying the effect of PAGGGM on RBC storage was studied in more detail By using double-erythrocytapheresis units (leukoreduced), a direct comparison could be made between the current AS saline-adenine-glucose-mannitol (SAGM) and the experimental solution PAGGGM During cold storage, several in vitro characteristics were analyzed.RESULTS: In agreement with our previous findings with single RBCs, PAGGGM maintained 2,3-DPG and ATP levels for 35 days of cold storage Furthermore, glucose consumption and lactate production were higher in PAGGGM units during the first 21 days of cold storage Fructose-1,6-diphophate and dihydroxyacetone phosphate levels were also increased during the first 21 days of storage in PAGGGM unitsCONCLUSION: These results indicate that it is likely that phosphofructokinase (PFK) activity is enhanced in PAGGGM units relative to SAGM units After 21 days, PFK activity also decreases in PAGGGM units, but sufficient metabolic reserve in these units prevents depletion of 2,3-DPG and ATP