Isolation and characterization of subgenomic DNAs encapsidated in "single" T=1 isometric particles of Maize streak virus
Isolation and characterization of subgenomic DNAs encapsidated in "single" T=1 isometric particles of Maize streak virus
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DOI：
10.1016/j.virol.2004.02.014
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发表时间：
2004-05-20
期刊：
影响因子：
3.7
通讯作者：
Boulton, MI
中科院分区：
文献类型：
--
作者：
Casado, CG;Ortiz, GJ;Boulton, MI
从受感染的玉米叶子中分离出“单一”T = 1 玉米条纹病毒 (MSV) 等距颗粒。生化和遗传特征表明，这些颗粒含有由 MSV 外壳蛋白包裹的亚基因组 (sg) MSV DNA。最大的 sg DNA 为 1.56 kb，略大于基因组大小的一半，尽管也克隆了小至 0.2 kb 的 sg DNA。 sg DNA 不具有传染性，并且它们似乎在 MSV 的致病性中不发挥作用。这是 MSV sg DNA 的首次报道，据我们所知，也是首次在任何双生病毒的序列水平上对衣壳化的 sg DNA 进行表征。这些数据将有助于我们研究基因组 DNA 在双生病毒科独特的双生衣壳结构形成中的作用。 (C) 2004 Elsevier Inc. 保留所有权利。
"Single" T = 1 isometric particles of Maize streak virus (MSV) have been isolated from infected maize leaves. Biochemical and genetic characterizations show that these particles contain subgenomic (sg) MSV DNA encapsidated by the MSV coat protein. The largest sg DNA is 1.56 kb, slightly larger than half genome size, although sg DNAs as small as 0.2 kb were also cloned. The sg DNAs are not infectious, and they do not appear to play a role in the pathogenicity of MSV. This is the first report of sg DNAs for MSV and, to our knowledge, the first time that encapsidated sg DNAs have been characterized at the sequence level for any geminivirus. These data will assist in our investigations into the role of genomic DNA in the formation of the unique geminate capsid architecture of the Geminiviridae. (C) 2004 Elsevier Inc. All rights reserved.