Characterization of a Hydrogel Derived from Decellularized Corneal Extracellular Matrix
Characterization of a Hydrogel Derived from Decellularized Corneal Extracellular Matrix
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DOI：
10.1166/jbt.2015.1410
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发表时间：
2015-12-01
影响因子：
0.1
通讯作者：
Fu, Yao
中科院分区：
文献类型：
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作者：
Lu, Yang;Yao, Qin Ke;Fu, Yao
在临床和临床前研究中，由细胞外基质(ECM)组成的生物支架常用于促进各种组织的创面愈合。近年来，ECM水凝胶因其组成可变、生物相容性好、能够填充不规则形状的空隙等优点而得到广泛应用。本研究的目的是对猪角膜基质(CM)水凝胶进行表征，并评价其对角膜基质细胞和角膜内皮细胞的体外增殖作用。结果表明，CM水凝胶保留了蛋白质和多肽的复杂的角膜ECM(CM)排列，并且在相同的ECM浓度下，这些CM水凝胶具有不同的组成和生物力学性能。此外，CM水凝胶具有良好的细胞相容性，可促进角膜基质细胞和角膜内皮细胞的体外增殖。CM水凝胶的独特组成和生物学特性表明，它可能在角膜再生中应用。
Biological scaffolds composed of extracellular matrix (ECM) are often used to facilitate wound healing in a variety of tissues in clinical and preclinical studies. Recently, ECM hydrogels have been widely used because of their advantages, such as a variable composition, good biocompatibility, and the ability to fill an irregularly shaped space. The objectives of the present study were to characterize a hydrogel from porcine corneal ECM (CM) and to evaluate the proliferation of keratocytes and corneal endothelial cells on the gel in vitro. The results showed that CM hydrogels retain a complex corneal ECM (CM) arrangement of proteins and peptides, and, at comparable ECM concentrations, these CM hydrogels have distinct compositions and biomechanical properties. Furthermore, the CM hydrogels are cytocompatible and promote keratocyte and corneal endothelial cell proliferation in vitro. The unique composition and biological properties of CM hydrogel suggest it may be useful for cornea regenerative applications.