Lipase-catalyzed synthesis of epigallocatechin gallate-based polymer for long-term release of epigallocatechin gallate with antioxidant property
Lipase-catalyzed synthesis of epigallocatechin gallate-based polymer for long-term release of epigallocatechin gallate with antioxidant property
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DOI：
10.1002/app.47693
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发表时间：
2019-07-10
影响因子：
3
通讯作者：
Iwamoto，Hiroyuki
中科院分区：
文献类型：
--
作者：
Nitta，Sachiko;Iwamoto，Hiroyuki
表没食子儿茶素没食子酸酯(EGCG)必须以受控的方式释放，以最大限度地发挥其作为抗氧化剂的长期潜力。在本研究中，通过脂肪酶催化EGCG与己二酸二乙烯酯和糖醇的聚合，形成聚EGCG，通过酯键的水解实现了EGCG的长期控制释放。聚合物的产率受单体配比、溶剂用量和脂肪酶浓度的影响。聚EGCG的酯基在两个多月的降解过程中，在一定的生理条件下逐渐释放，释放速率受糖醇类型和EGCG比例的影响。聚表没食子儿茶素没食子酸酯释放的EGCG保持了其抗氧化活性，当聚合物浓度大于0.0 5 mg/mL时，细胞存活率高达10 0%。这些结果表明，多聚EGCG作为一种高效、长效、低细胞毒性的抗氧化材料具有潜在的应用前景。2019威利期刊公司J.应用。波兰姆。科学2019,136,47693。
Epigallocatechin gallate (EGCG) must be released in a controlled manner in order to maximize its long‐term potential as an antioxidant. In this study, lipase‐catalyzed polymerization of EGCG with divinyl adipate and sugar alcohols was conducted to form polyEGCG, which enables long‐term controlled release of EGCG via hydrolysis of ester linkages. The polymer yields were influenced by monomer ratios, volume of solvent, and lipase concentration. EGCG was gradually released from polyEGCG under a physiological condition as ester groups of polyEGCG degraded over a period of more than two months, and the releasing rate was influenced by the type of the sugar alcohols and EGCG ratios. EGCG released from polyEGCG maintained its antioxidant activity and the cell viability of polyEGCG eluent with polymer concentration higher than 0.05 mg/mL was as high as 100%. These results indicate that polyEGCG has potential application as a highly efficient and long‐term antioxidative material with low cytotoxicity. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2019,136, 47693.