HISTOLOGICAL CHANGES IN RAT LIVER AFTER CHRONIC IRON-SORBITOL OVERLOAD
HISTOLOGICAL CHANGES IN RAT LIVER AFTER CHRONIC IRON-SORBITOL OVERLOAD
复制标题
慢性铁山梨醇超载后大鼠肝脏的组织学变化
DOI：
10.1501/jms_0000000012
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发表时间：
2005
影响因子：
7.3
通讯作者：
M. Özgüner
中科院分区：
文献类型：
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作者：
M. Özgüner
------------------------- ----------------------------------------------------------------------------------------------------铁是一种在血红素代谢中起重要作用的金属，本研究通过建立慢性铁-山梨醇超负荷大鼠模型，观察了铁-山梨醇超负荷后的组织学变化。通过肌肉内给予10只SpragueDawley大鼠（200-250 gr）0.4 mg/g/天的铁-山梨醇，每周两天，持续8周，产生了实验性铁过载。对照组由注射生理盐水的10只Sprague-Dawley大鼠组成。肝组织切片经苏木精-伊红、珀尔斯普鲁士蓝、马森三色染色和过碘酸希夫（PAS）试剂染色。显微镜下发现窦状隙细胞和实质细胞充满铁沉积物，观察到轻度纤维化伴弥漫性白细胞浸润。本实验研究表明，在我国唯一的商业胃肠外形式的铁，铁山梨醇（Jectofer），可以产生形态学的变化，在一个相对较短的时间内，该模型可能是有用的，为进一步的肠外铁过载的研究在未来。总之，本研究揭示了慢性铁毒性在肝脏中的认识，并且进一步的努力应针对慢性铁过载的并发症和组织学发现的相关性。
––––––––––––––––––––––––– –––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––––– SUMMARY Iron is a metal that plays an important role in heme metabolism.This study revealed the histological changes in a new rat model after chronic iron-sorbitol overload. Experimental iron overload has been produced by intramuscular administration of iron-sorbitol 0.4 mg/g/day for two days per week, for eight weeks to 10 SpragueDawley rats(200-250 gr). The control group consisted of 10 Sprague-Dawley rats injected with saline. Histological sections from liver stained by hematoxylin-eosin, Perls’ Prussian blue, Masson’s trichrome stain and periodic acid Schiff (PAS) reagent. Microscopical findings revealed that sinusoidal cells and parenchymal cells were filled with iron deposits, and a slight fibrosis was observed with diffuse leucocyte infiltration. This experimental study revealed that the only commercial parenteral form of iron in our country, iron-sorbitol (Jectofer), could produce morphologic changes in a relatively short period of time and this model could be useful for further parenteral iron overload investigations in the future. On the whole, this study revealed to be aware of chronic iron toxicity in liver and further efforts should be directed toward the complication of chronic iron overload and the correlation of histological findings.
大鼠膳食羰基铁超负荷的病理学。
DOI：
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发表时间：
1987
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Park,CH;Bacon,BR;Brittenham,GM;Tavill,AS
通讯作者：
Tavill,AS
影响因子：
--
作者：
R. Britton;G. Ramm;J. Olynyk;R. Singh;R. O'Neill;B. Bacon
通讯作者：
R. Britton;G. Ramm;J. Olynyk;R. Singh;R. O'Neill;B. Bacon
影响因子：
3.1
作者：
BONKOVSKY, HL
通讯作者：
BONKOVSKY, HL