Design of a high-throughput assay for alternative splicing using polymerase colonies.
Design of a high-throughput assay for alternative splicing using polymerase colonies.
复制标题
使用聚合酶集落进行选择性剪接的高通量测定的设计。
DOI：
10.1142/9789812704856_0002
复制
发表时间：
2004
影响因子：
--
通讯作者：
Mitra,RD
中科院分区：
文献类型：
--
作者：
Buhler,JD;Souvenir,RM;Zhang,W;Mitra,RD
We propose an assay to detect and quantify alternative splicing simultaneously for numerous genes in a pool of cellular mRNA. The assay exploitspolymerase colonies, a recently developed method for sampling and amplifying large numbers of individual transcript molecules into discrete spots on a gel. The proposed assay combines the advantages of microarrays for transcript quantitation with the sensitivity and precision of methods based on counting single transcript molecules. Given a collection of spots si, each containing an unknown splice variant of some known gene Gi, we design a series of hybridizations to short oligonucleotide probes to determine in parallel which exons of Giare present in every spot si. We give algorithms to minimize the cost of such designs.
