Genome-wide distribution and potential regulatory functions of AtATE, a novel family of miniature inverted-repeat transposable elements in Arabidopsis thaliana
Genome-wide distribution and potential regulatory functions of AtATE, a novel family of miniature inverted-repeat transposable elements in Arabidopsis thaliana
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DOI：
10.1007/s00438-002-0675-4
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Couée, I
中科院分区：
文献类型：
--
作者：
El Amrani, A;Marie, L;Couée, I
对转基因启动子：：β-葡萄糖醛酸苷酶的研究表明，两个拟南芥精氨酸脱羧酶类似物ADC1和ADC2的启动子表现出极不同的活性模式。ADC1启动子的一个主要特征是存在一个新的转座元件，它具有微型反向重复转座元件(METE)的所有特征，存在于26个全长拷贝和1617个部分拷贝和片段中，分布在拟南芥基因组中。TRANSFAC分析表明，该转座元件具有大量的转录因子结合基序。基于后缀树汇编的生物信息学方法被用来获得该元件在拟南芥基因组中的确切拷贝数和位置的详尽描述。染色体之间的分布是非随机的，在基因两侧的区域发现了大量的拷贝。在22个基因的上游或下游的附近检测到了转座元件的全长拷贝。
A study of transgenic promoter::beta-glucuronidase lines showed that the promoters of the two Arabidopsis ARGININE DECARBOXYLASE paralogues, ADC1 and ADC2, exhibited extremely different patterns of activity. One major feature of the promoter of ADC1 was the presence of a novel transposable element, which was shown to possess all of the characteristics of Miniature Inverted-repeat Transposable Elements (MITEs), and to be present in 26 full-length copies and 1617 partial copies and fragments distributed throughout the Arabidopsis genome. TRANSFAC analysis showed that this transposable element possesses a significant number of transcription-factor binding motifs. A bioinformatics approach based on a suffix-tree compilation was used to obtain an exhaustive description of exact copy numbers and positions of the element in the Arabidopsis genome. The distribution among the chromosomes was non-random, and a significant number of copies were found in regions flanking genes. Full-length copies of the transposable element were detected in the immediate vicinity of 22 genes, either upstream or downstream.