Effect of urban traffic, individual habits, and genetic polymorphisms on background urinary 1-hydroxypyrene excretion
Effect of urban traffic, individual habits, and genetic polymorphisms on background urinary 1-hydroxypyrene excretion
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DOI：
10.1016/j.annepidem.2005.11.001
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发表时间：
2007-01-01
影响因子：
5.6
通讯作者：
Flore, Costantino
中科院分区：
文献类型：
--
作者：
Cocco, Pierluigi;Moore, Patrick S.;Flore, Costantino
目得：多环芳烃（PAHs）暴露的潜在来源和基因多态性进行了调查，其贡献的个体间变异的基线水平的尿1-羟基芘（1-OHP）排泄在受试者中没有职业暴露于PAHs.METHODS：尿1-OHP的排泄量进行了测定114人，其中包括48名女性和66名男性。调查问卷信息收集可能的环境和个人来源的多环芳烃暴露。一个70人的子集也进行了评估的单核苷酸多态性结果：1-OHP值无明显的季节性变化，且不受年龄、文化程度、体重指数、性别、年龄、性别、年龄、性别、性别、年龄、性别吸烟状态，包括被动吸烟;或CYP 1A 2基因外显子7第295位C -> T碱基取代。经Logistic回归相互校正后，生活在交通繁忙的城市地区（比值比，4.9; 95%置信区间，1.0-24.9）和经常摄入烤肉（比值比，6.9; 95%置信区间，1.1-43.5）是背景尿1-OHP水平为0.50 μ g/g肌酐或更高的显著预测因素。升高的风险也与每日酒精摄入量大于65克和nonnull GSTT 1 genotype.CONCLUSION：我们的研究表明，暴露于城市交通，饮食习惯，和nonnull GSTT 1基因型可能有助于个体间的差异，在没有职业暴露于多环芳烃的受试者的背景水平的1-OHP尿排泄。
PURPOSE: Potential sources of exposure to polycyclic aromatic hydrocarbons (PAHs) and genetic polymorphisms were investigated in relation to their contribution to interindividual variation in baseline levels of urinary 1-hydroxypyrene (1-OHP) excretion in subjects without occupational exposure to PAHs.METHODS: Urinary excretion of 1-OHP was measured in 114 subjects, including 48 women and 66 men. Questionnaire information was collected on possible environmental and individual sources of PAH exposure. A subset of 70 individuals also was evaluated for a single-nucleotide polymorphism (Ex7 + 295C -> T) in the cytochrome P-450 1A2 (CYP1A2) gene, and 61 of these also were evaluated for the glutathione transferase T1 (GSTT1) gene polymorphism.RESULTS: 1-OHP values did not show a significant seasonal variability and were unaffected by age; education; body mass index; smoking status, including passive smoking; or the C -> T base substitution in position 295 of exon 7 of the CYP1A2 gene. After reciprocal adjustment with logistic regression, living in a heavily trafficked urban area (odds ratio, 4.9; 95% confidence interval, 1.0-24.9), and frequent intake of grilled meat (odds ratio, 6.9; 95% confidence interval, 1.1-43.5) were significant predictors of background urinary 1-OHP levels of 0.50 mu g/g creatinine or greater. Elevated risks also were associated with daily alcohol intake greater than 65 g and the nonnull GSTT1 genotype.CONCLUSION: Our study shows that exposure to urban traffic, dietary habits, and the nonnull GSTT1 genotype may contribute to interindividual variation in background levels of 1-OHP urinary excretion in subjects without occupational exposure to PAHs.