Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.
Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.
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使用淬灭荧光底物测定脑组织和脑脊液中血管紧张素转换酶 2 (ACE2) 的酶活性。
DOI：
10.1007/978-1-4939-6625-7_9
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lazartigues,Eric
中科院分区：
文献类型：
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作者：
Sriramula,Srinivas;Pedersen,KimBrint;Xia,Huijing;Lazartigues,Eric
血管紧张素转换酶 2 (ACE2) 是肾素-血管紧张素系统 (RAS) 的组成部分，在血压和容量稳态的调节中发挥着重要作用。越来越多的证据表明，几种高血压动物模型以及高血压患者的 ACE2 表达和活性发生了变化。为了评估大脑 ACE2 在高血压中的作用，需要进行特定的 ACE2 检测。基于猝灭荧光底物，我们描述了一种易于使用的方法来测定脑组织和脑脊液中的 ACE2 活性。该方法还可适用于其他组织、血浆、细胞提取物和细胞培养物上清液。
Angiotensin-converting enzyme 2 (ACE2) is a component of the renin-angiotensin system (RAS) which plays an important role in the regulation of blood pressure and volume homeostasis. Accumulating evidence shows alterations in ACE2 expression and activity in several hypertensive animal models, as well as in patients with hypertension. In order to assess the role of brain ACE2 in hypertension, a specific ACE2 assay is required. Based on a quenched fluorescent substrate, we describe an easy-to-use method for determining ACE2 activity in brain tissue and cerebrospinal fluid. The method can further be adapted for other tissues, plasma, cell extracts, and cell culture supernatants.