THE SPACING OF ESCHERICHIA-COLI DNA GYRASE SITES CLEAVED INVIVO BY TREATMENT WITH OXOLINIC ACID AND SODIUM DODECYL-SULFATE
THE SPACING OF ESCHERICHIA-COLI DNA GYRASE SITES CLEAVED INVIVO BY TREATMENT WITH OXOLINIC ACID AND SODIUM DODECYL-SULFATE
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DOI：
10.1016/0300-9084(84)90258-x
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发表时间：
1984-01-01
期刊：
影响因子：
3.9
通讯作者：
BOUCHE, JP
中科院分区：
文献类型：
--
作者：
BEJAR, S;BOUCHE, JP
为了在大肠杆菌染色体的特定区域中定位旋转酶结合位点，人们重新研究了奥索林酸和十二烷基硫酸钠促进旋转酶对染色体 DNA 的切割。之前的一份报告表明每 100 kb [千碱基] DNA 存在 1 个位点，与此相反，我们发现每 25 或 12 kb 就有一个切割频率，具体取决于生长培养基。通过Southern印迹杂交寻找切割位点未能揭示任何高频率切割的结合位点。位点的实际间距可能比根据切割频率确定的间距要近得多。对含有特定 DNA 序列的片段平均大小的测量表明，位点频率沿染色体变化。与oriC相对的区域位点相对较少。
In an attempt to locate gyrase binding sites in a specific region of the chromosome of E. coli, one reinvestigated gyrase-promoted cleavage of chromosomal DNA by oxolinic acid and sodium dodecyl sulfate. Contrary to a previous report suggesting the presence of 1 site every 100 kb [kilobases] of DNA one found frequencies of one cleavage every 25 or 12 kb, depending on the growth medium. A search for cleavage sites by Southern blot hybridization failed to reveal any binding site cleaved at a high frequency. The actual spacing of sites may be much closer than that determined from the frequency of cleavage. Measurement of the average size of fragments containing defined DNA sequences indicated that the frequency of sites varies along the chromosome. The region located opposite to oriC carries relatively few sites.