Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma
Insulin-like growth factor binding protein-3 gene methylation and protein expression in gastric adenocarcinoma
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DOI：
10.1016/j.ghir.2010.02.005
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发表时间：
2010-06-01
影响因子：
1.4
通讯作者：
Cardoso Smith, Marilia de Arruda
中科院分区：
文献类型：
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作者：
Gigek, Carolina Oliveira;Leal, Mariana Ferreira;Cardoso Smith, Marilia de Arruda
目的：探讨IGFBP-3蛋白在胃癌发生过程中的表达、与基因启动子甲基化模式的关系及与临床病理特征的关系。设计：对43例正常胃黏膜和94例腺癌标本进行亚硫酸氢盐修饰后的甲基化特异性PCR检测。对54例胃癌和20例正常胃粘膜标本进行过氧化物酶免疫组化分析。结果：IGFBP-3在肿瘤组织中的表达高于正常粘膜组织（p < 0.0001）。肠型的蛋白表达频率高于弥漫型（p = 0.0412）。胃样本中IGFBP-3启动子甲基化频率在肿瘤和非肿瘤样本中分别为95.7%和97.7%。IGFBP-3甲基化的频率在肿瘤和正常样本之间没有差异（95.7%对97.7%,p = 0.7810）。我们没有观察到IGFBP-3启动子甲基化与蛋白表达之间的显著相关性。结论：综上所述，本研究未观察到IGFBP-3启动子甲基化对蛋白表达的影响。此外，我们提出胃组织IGFBP-3免疫染色可能是一个有用的恶性肿瘤标志物。(C) 2010 Elsevier Ltd.版权所有。
Objective: The aim of this study was to evaluate IGFBP-3 protein expression, its correlation with gene promoter methylation pattern in gastric carcinogenesis and with clinicopathological characteristics.Design: Forty-three normal gastric mucosa and 94 adenocarcinoma samples were investigated through methylation specific PCR, after bisulfite modification. Immunohistochemistry was analyzed using peroxidase in 54 gastric cancer and 20 normal gastric mucosa samples.Results: IGFBP-3 expression was higher in tumor samples than in normal mucosa (p < 0.0001). Intestinal type presented a higher frequency of protein expression than diffuse type (p = 0.0412). Methylation frequency of IGFBP-3 promoter in gastric samples revealed, respectively, 95.7% and 97.7% in neoplastic and non-neoplastic samples. The frequency of IGFBP-3 methylation did not differ between tumor and normal samples (95.7% versus 97.7%, p = 0.7810). We did not observe a significant correlation between IGFBP-3 promoter methylation and protein expression.Conclusion: In summary, our study did not observe any influence of IGFBP-3 promoter methylation on protein expression. Moreover we propose that IGFBP-3 immunostaining in gastric tissue may be a useful marker for malignancy. (C) 2010 Elsevier Ltd. All rights reserved.