cDNA clones encoding bovine gamma-crystallins.
cDNA clones encoding bovine gamma-crystallins.
复制标题
编码牛γ-晶状体蛋白的cDNA克隆。
DOI：
10.1016/0006-291x(87)90729-7
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发表时间：
1987
影响因子：
3.1
通讯作者：
Petrash,JM
中科院分区：
文献类型：
--
作者：
Hay,RE;Woods,WD;Church,RL;Petrash,JM
我们测定了两个牛晶状体γ-晶体蛋白cDNA克隆pBLγII-1和pBLγIII-1的核苷酸序列。pBLγII-1全长644 bp的cDNA插入片段包含牛γ ii -晶体蛋白整个氨基酸序列的编码信息。pBLγIII-1的497 bp cDNA插入片段编码同源但不同的γ-晶体蛋白多肽，并且缺乏c端17个氨基酸残基的编码信息。克隆编码区的核苷酸序列和预测氨基酸序列具有高度的同源性，而未翻译先导序列则存在较大差异。pBLγIII-1的先导序列与兔免疫球蛋白α-重链mRNA的一部分惊人地同源。
We have determined the nucleotide sequence of two bovine lens γ-crystallin cDNA clones, pBLγII-1 and pBLγIII-1. The 644 bp cDNA insert of pBLγII-1 contains coding information for the entire amino acid sequence of bovine γII-crystallin. The 497 bp cDNA insert of pBLγIII-1 encodes a homologous but different γ-crystallin polypeptide, and appears to lack the coding information for the C-terminal 17 amino acid residues. While the nucleotide and predicted amino acid sequences of the coding regions of the clones show a high degree of homology, the untranslated leader sequences are relatively dissimilar. The leader sequence of pBLγIII-1 is strikingly homologous to a portion of a rabbit immunoglobulin α-heavy chain mRNA.