Comparison of multivariate analysis methods for extracting the paraffin component from the paraffin-embedded cancer tissue spectra for Raman imaging.
Comparison of multivariate analysis methods for extracting the paraffin component from the paraffin-embedded cancer tissue spectra for Raman imaging.
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DOI：
10.1038/srep44890
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发表时间：
2017-03-22
影响因子：
4.6
通讯作者：
Ozaki Y
中科院分区：
文献类型：
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作者：
Meksiarun P;Ishigaki M;Huck-Pezzei VA;Huck CW;Wongravee K;Sato H;Ozaki Y
本研究旨在使用三种多变量分析（MVA）方法从石蜡包埋的口腔癌组织光谱中提取石蜡成分;独立成分分析（伊卡）、偏最小二乘法（PLS）和独立成分-偏最小二乘法（IC-PLS）。估计的石蜡组分用于从组织光谱中去除石蜡的贡献。比较了这三种方法的除蜡效率和保留组织信息的能力。结果表明，伊卡、PLS和IC-PLS对烷烃组分的去除效果基本相同，而主成分分析（PCA）则不能。在保留癌组织光谱完整性方面，PLS和IC-PLS对非石蜡区域的影响显著小于伊卡，其中癌组织光谱区域恶化。将去除石蜡的光谱用于构建口腔癌组织的拉曼图像，并与苏木精和伊红（H&E）染色的组织进行比较以进行验证。本研究证明了拉曼光谱与多元分析方法一起作为石蜡包埋组织切片的诊断工具的能力。
This study aimed to extract the paraffin component from paraffin-embedded oral cancer tissue spectra using three multivariate analysis (MVA) methods; Independent Component Analysis (ICA), Partial Least Squares (PLS) and Independent Component - Partial Least Square (IC-PLS). The estimated paraffin components were used for removing the contribution of paraffin from the tissue spectra. These three methods were compared in terms of the efficiency of paraffin removal and the ability to retain the tissue information. It was found that ICA, PLS and IC-PLS could remove the paraffin component from the spectra at almost the same level while Principal Component Analysis (PCA) was incapable. In terms of retaining cancer tissue spectral integrity, effects of PLS and IC-PLS on the non-paraffin region were significantly less than that of ICA where cancer tissue spectral areas were deteriorated. The paraffin-removed spectra were used for constructing Raman images of oral cancer tissue and compared with Hematoxylin and Eosin (H&E) stained tissues for verification. This study has demonstrated the capability of Raman spectroscopy together with multivariate analysis methods as a diagnostic tool for the paraffin-embedded tissue section.